GUCY1B3 Recombinant Rabbit Monoclonal Antibody [JE41-09]
Catalog# HA722314
GUCY1B3 Recombinant Rabbit Monoclonal Antibody [JE41-09]
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WB
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Human
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Mouse
-
Rat
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unconjugated
概述
产品名称
GUCY1B3 Recombinant Rabbit Monoclonal Antibody [JE41-09]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein within Human GUCY1B3 aa 1-100 / 619.
种属反应性
Human, Mouse, Rat
验证应用
WB
分子量
Predicted band size: 71 kDa
阳性对照
Human lung tissue lysate, human brain tissue lysate, mouse lung tissue lysate, mouse brain tissue lysate, mouse spleen tissue lysate, rat brain tissue lysate, rat spleen tissue lysate.
偶联
unconjugated
克隆号
JE41-09
反应性数据
| WB | |
|---|---|
| Human |
|
| Mouse |
|
| Rat |
|
产品特性
形态
Liquid
浓度
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
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1:1,000
靶点
功能
Guanylate cyclase soluble subunit beta-1 is an enzyme that in humans is encoded by the GUCY1B3 gene. Soluble guanylate cyclase (sGC), a heterodimeric protein consisting of an alpha and a beta subunit, catalyzes the conversion of GTP to the second messenger cGMP and functions as the main receptor for nitric oxide and nitrovasodilator drugs. GUCY1B3 has been shown to interact with Heat shock protein 90kDa alpha (cytosolic), member A1 and Endothelial NOS.
背景文献
1. Korkmaz Y et al. Dental Pulp Inflammation Initiates the Occurrence of Mast Cells Expressing the alpha(1) and beta(1) Subunits of Soluble Guanylyl Cyclase. Int J Mol Sci. 2023 Jan
2. Vishnolia KK et al. sGC Activity and Regulation of Blood Flow in a Zebrafish Model System. Front Physiol. 2021 Feb
亚细胞定位
Cytoplasm.
别名
GC-S-beta-1 antibody
GC-SB3 antibody
GCbeta1 antibody
GCS beta 1 antibody
GCS beta 3 antibody
GCS-beta-1 antibody
GCS-beta-3 antibody
GCYB1_HUMAN antibody
guanylate cyclase 1, soluble, beta 3 antibody
Guanylate cyclase soluble beta 1 chain antibody
展开图片
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Western blot analysis of GUCY1B3 on different lysates with Rabbit anti-GUCY1B3 antibody (HA722314) at 1/1,000 dilution.
Lane 1: Human lung tissue lysate
Lane 2: Human brain tissue lysate
Lane 3: Mouse lung tissue lysate
Lane 4: Mouse brain tissue lysate
Lane 5: Mouse spleen tissue lysate
Lane 6: Rat brain tissue lysate
Lane 7: Rat spleen tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 71 kDa
Observed band size: 71 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722314) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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