Mouse IL-17A Recombinant Rabbit Monoclonal Antibody [PSH05-35] - BSA and Azide free (Capture)
概述
产品名称
Mouse IL-17A Recombinant Rabbit Monoclonal Antibody [PSH05-35] - BSA and Azide free (Capture)
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein within mouse IL-17A aa 26-158 (Q62386).
种属反应性
Mouse, Rat
验证应用
ELISA(Cap)
阳性对照
Recombinant standard Mouse IL-17A protein (HA210791).
偶联
unconjugated
克隆号
PSH05-35
产品特性
形态
Liquid
浓度
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
PBS (pH7.4).
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
ELISA(Cap)
-
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH05-36] to Mouse IL-17A (Detector) (HA722265) and recombinant standard Mouse IL-17A protein (HA210791). The reference range value is 9.9-800pg/ml.
靶点
功能
Effector cytokine of innate and adaptive immune system involved in antimicrobial host defense and maintenance of tissue integrity. Signals via IL17RA-IL17RC heterodimeric receptor complex, triggering homotypic interaction of IL17RA and IL17RC chains with TRAF3IP2 adapter. This leads to downstream TRAF6-mediated activation of NF-kappa-B and MAPkinase pathways ultimately resulting in transcriptional activation of cytokines, chemokines, antimicrobial peptides and matrix metalloproteinases, with potential strong immune inflammation. Plays an important role in connecting T cell-mediated adaptive immunity and acute inflammatory response to destroy extracellular bacteria and fungi. As a signature effector cytokine of T-helper 17 cells (Th17), primarily induces neutrophil activation and recruitment at infection and inflammatory sites. In airway epithelium, mediates neutrophil chemotaxis via induction of CXCL1 and CXCL5 chemokines. In secondary lymphoid organs, contributes to germinal center formation by regulating the chemotactic response of B cells to CXCL12 and CXCL13, enhancing retention of B cells within the germinal centers, B cell somatic hypermutation rate and selection toward plasma cells. Effector cytokine of a subset of gamma-delta T cells that functions as part of an inflammatory circuit downstream IL1B, TLR2 and IL23A-IL12B to promote neutrophil recruitment for efficient bacterial clearance.
背景文献
1. Wu M et al. Epithelium-derived IL17A Promotes Cigarette Smoke-induced Inflammation and Mucus Hyperproduction. Am J Respir Cell Mol Biol. 2021 Dec
2. Li Y et al. Neutrophils and IL17A mediate flagellar hook protein FlgE-induced mouse acute lung inflammation. Cell Microbiol. 2019 Mar
亚细胞定位
Secreted.
UNIPROT
别名
CTLA 8 antibody
CTLA-8 antibody
CTLA8 antibody
cytotoxic T lymphocyte associated antigen 8 antibody
Cytotoxic T lymphocyte associated protein 8 antibody
Cytotoxic T lymphocyte associated serine esterase 8 antibody
Cytotoxic T-lymphocyte-associated antigen 8 antibody
IL 17 antibody
IL 17A antibody
IL-17 antibody
展开图片
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![Sandwich ELISA analysis of Mouse IL-17A matched pair antibodies<br /><br />Capture: <a href="/products/HA722264" style="font-weight: bold;text-decoration: underline;">HA722264</a>, Mouse IL-17A Rabbit mAb [PSH05-35]<br />Detector: <a href="/products/HA722265" style="font-weight: bold;text-decoration: underline;">HA722265</a>, Mouse IL-17A Rabbit mAb [PSH05-36]<br /><br />Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (<a href="/products/HA722264" style="font-weight: bold;text-decoration: underline;">HA722264</a>) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted recombinant standard Mouse IL-17A protein (<a href="/products/HA210791" style="font-weight: bold;text-decoration: underline;">HA210791</a>) starting from 800 pg/ml to 0 pg/ml and detect antibody (<a href="/products/HA722265" style="font-weight: bold;text-decoration: underline;">HA722265</a>, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.](https://storage.huabio.cn/huabio/productImg/HA722264_1.png?v=20260310114549)
Sandwich ELISA analysis of Mouse IL-17A matched pair antibodies
Capture: HA722264, Mouse IL-17A Rabbit mAb [PSH05-35]
Detector: HA722265, Mouse IL-17A Rabbit mAb [PSH05-36]
Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA722264) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted recombinant standard Mouse IL-17A protein (HA210791) starting from 800 pg/ml to 0 pg/ml and detect antibody (HA722265, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. -
![Mouse lung were stimulated with 5 µg/mL LPS and incubated for 6 days.<br /><br />Capture: <a href="/products/HA722264" style="font-weight: bold;text-decoration: underline;">HA722264</a>, Mouse IL-17A Rabbit mAb [PSH05-35]<br />Detector: <a href="/products/HA722265" style="font-weight: bold;text-decoration: underline;">HA722265</a>, Mouse IL-17A Rabbit mAb [PSH05-36]<br /><br />The concentrations of IL17A were interpolated from the IL17A standard curves and corrected for sample dilution.Undiluted samples are LPS stimulated mouse lung supernatants 50% and unstimulated mouse lung supernatants 50%. The mean IL17A concentration was determined to be 621 pg/mL in LPS stimulated mouse lung supernatant and undetectable in the unstimulated mouse lung supernatant.](https://storage.huabio.cn/huabio/productImg/HA722264_2.png?v=20260310114549)
Mouse lung were stimulated with 5 µg/mL LPS and incubated for 6 days.
Capture: HA722264, Mouse IL-17A Rabbit mAb [PSH05-35]
Detector: HA722265, Mouse IL-17A Rabbit mAb [PSH05-36]
The concentrations of IL17A were interpolated from the IL17A standard curves and corrected for sample dilution.Undiluted samples are LPS stimulated mouse lung supernatants 50% and unstimulated mouse lung supernatants 50%. The mean IL17A concentration was determined to be 621 pg/mL in LPS stimulated mouse lung supernatant and undetectable in the unstimulated mouse lung supernatant. -
![Interpolated concentrations of native IL17A in unstimulated or stimulated rat spleen culture supernatant.<br /><br />Capture: <a href="/products/HA722264" style="font-weight: bold;text-decoration: underline;">HA722264</a>, Mouse IL-17A Rabbit mAb [PSH05-35]<br />Detector: <a href="/products/HA722265" style="font-weight: bold;text-decoration: underline;">HA722265</a>, Mouse IL-17A Rabbit mAb [PSH05-36]<br /><br />Rat spleen were stimulated with 5 µg/mL Concanavalin A and incubated for 2 days.The concentrations of IL17A were interpolated from the IL17A standard curves and corrected for sample dilution. Undiluted samples are Concanavalin A stimulated rat spleen supernatants 20% and unstimulated rat spleen supernatants 50%. The mean IL17A concentration was determined to be 3,703 pg/mL in Concanavalin A stimulated rat spleen supernatant and undetectable in the unstimulated rat spleen supernatant.](https://storage.huabio.cn/huabio/productImg/HA722264_3.png?v=20260310114549)
Interpolated concentrations of native IL17A in unstimulated or stimulated rat spleen culture supernatant.
Capture: HA722264, Mouse IL-17A Rabbit mAb [PSH05-35]
Detector: HA722265, Mouse IL-17A Rabbit mAb [PSH05-36]
Rat spleen were stimulated with 5 µg/mL Concanavalin A and incubated for 2 days.The concentrations of IL17A were interpolated from the IL17A standard curves and corrected for sample dilution. Undiluted samples are Concanavalin A stimulated rat spleen supernatants 20% and unstimulated rat spleen supernatants 50%. The mean IL17A concentration was determined to be 3,703 pg/mL in Concanavalin A stimulated rat spleen supernatant and undetectable in the unstimulated rat spleen supernatant.
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