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GRP78 / BIP Recombinant Rabbit Monoclonal Antibody [JE01-35]

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Catalog# HA722202

GRP78 / BIP Recombinant Rabbit Monoclonal Antibody [JE01-35]

Application

  • WB

  • IHC-P

  • IF-Tissue

Reactivity

  • Human

  • Mouse

  • Rat

Predicted reactivity

  • Chicken

预测的种属支持售后
This product has been cited in peer reviewed publications, see list HERE

概述

产品名称

GRP78 / BIP Recombinant Rabbit Monoclonal Antibody [JE01-35]

抗体类型

Recombinant Rabbit monoclonal Antibody

免疫原

Recombinant protein within Human GRP78 / BIP aa 1-100 / 654.

种属反应性

Human, Mouse, Rat (Predicted: Chicken)

预测的种属支持售后

验证应用

WB, IHC-P, IF-Tissue

分子量

Predicted band size: 72 kDa

阳性对照

HeLa cell lysate, HepG2 cell lysate, MCF7 cell lysate, MDA-MB-231 cell lysate, A549 cell lysate, mouse liver tissue lysate, rat liver tissue lysate, human breast cancer tissue, human brain tissue, mouse brain tissue, rat brain tissue.

偶联

unconjugated

克隆号

JE01-35

产品特性

形态

Liquid

浓度

1ug/ul

存放说明

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

存储缓冲液

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

亚型

IgG

纯化方式

Protein A affinity purified.

应用稀释度

  • WB

  • 1:1,000

  • IHC-P

  • 1:1,000

  • IF-Tissue

  • 1:200

发表文章中的应用

WB 查看 2 篇文献如下

靶点

功能

Binding immunoglobulin protein (BiPS) also known as 78 kDa glucose-regulated protein (GRP-78) or heat shock 70 kDa protein 5 (HSPA5) is a protein that in humans is encoded by the HSPA5 gene. BiP is a HSP70 molecular chaperone located in the lumen of the endoplasmic reticulum (ER) that binds newly synthesized proteins as they are translocated into the ER, and maintains them in a state competent for subsequent folding and oligomerization. BiP is also an essential component of the translocation machinery and plays a role in retrograde transport across the ER membrane of aberrant proteins destined for degradation by the proteasome. BiP is an abundant protein under all growth conditions, but its synthesis is markedly induced under conditions that lead to the accumulation of unfolded polypeptides in the ER.

背景文献

1. Xia S et al. GRP78 in lung cancer. J Transl Med. 2021 Mar

2. Pan D et al. GRP78 Activity Moderation as a Therapeutic Treatment against Obesity. Int J Environ Res Public Health. 2022 Nov

亚细胞定位

Endoplasmic reticulum lumen, Melanosome, Cytoplasm, Cell surface.

UNIPROT

SwissProt: P11021 Human

SwissProt: P20029 Mouse

SwissProt: P06761 Rat

别名

78 kDa glucose regulated protein antibody

78 kDa glucose-regulated protein antibody

AL022860 antibody

AU019543 antibody

BIP antibody

D2Wsu141e antibody

D2Wsu17e antibody

Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78 antibody

Endoplasmic reticulum lumenal Ca2+ binding protein grp78 antibody

Epididymis secretory sperm binding protein Li 89n antibody

展开

图片

  • Western blot analysis of GRP78 / BIP on different lysates with Rabbit anti-GRP78 / BIP antibody (<a href="/products/HA722202" style="font-weight: bold;text-decoration: underline;">HA722202</a>) at 1/1,000 dilution.<br /><br />Lane 1: HeLa cell lysate (20 µg/Lane)<br />Lane 2: HepG2 cell lysate (20 µg/Lane)<br />Lane 3: MCF7 cell lysate (20 µg/Lane)<br />Lane 4: MDA-MB-231 cell lysate (20 µg/Lane)<br />Lane 5: A549 cell lysate (20 µg/Lane)<br />Lane 6: Mouse liver tissue lysate (40 µg/Lane)<br />Lane 7: Rat liver tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 72 kDa<br />Observed band size: 72 kDa<br /><br />Exposure time: 1 minute 16 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722202" style="font-weight: bold;text-decoration: underline;">HA722202</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of GRP78 / BIP on different lysates with Rabbit anti-GRP78 / BIP antibody (HA722202) at 1/1,000 dilution.

    Lane 1: HeLa cell lysate (20 µg/Lane)
    Lane 2: HepG2 cell lysate (20 µg/Lane)
    Lane 3: MCF7 cell lysate (20 µg/Lane)
    Lane 4: MDA-MB-231 cell lysate (20 µg/Lane)
    Lane 5: A549 cell lysate (20 µg/Lane)
    Lane 6: Mouse liver tissue lysate (40 µg/Lane)
    Lane 7: Rat liver tissue lysate (40 µg/Lane)

    Predicted band size: 72 kDa
    Observed band size: 72 kDa

    Exposure time: 1 minute 16 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722202) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-GRP78 / BIP antibody (<a href="/products/HA722202" style="font-weight: bold;text-decoration: underline;">HA722202</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722202" style="font-weight: bold;text-decoration: underline;">HA722202</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-GRP78 / BIP antibody (HA722202) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722202) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-GRP78 / BIP antibody (<a href="/products/HA722202" style="font-weight: bold;text-decoration: underline;">HA722202</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722202" style="font-weight: bold;text-decoration: underline;">HA722202</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-GRP78 / BIP antibody (HA722202) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722202) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-GRP78 / BIP antibody (<a href="/products/HA722202" style="font-weight: bold;text-decoration: underline;">HA722202</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722202" style="font-weight: bold;text-decoration: underline;">HA722202</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-GRP78 / BIP antibody (HA722202) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722202) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-GRP78 / BIP antibody (<a href="/products/HA722202" style="font-weight: bold;text-decoration: underline;">HA722202</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722202" style="font-weight: bold;text-decoration: underline;">HA722202</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-GRP78 / BIP antibody (HA722202) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722202) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Application: WB<br /><br />Species: Chicken<br /><br />Sample: Chest skin samples (CSFs)<br /><br />Antibody concentration: 1/1,000<br /><br />Date by conrtesy of: Mrs. Mei Jin, Liaoning Provincial Key Laboratory of Biotechnology and Drug Discovery, Liaoning Normal University<br /><br />Title: MicroRNAs involved in the toxic effects of sodium arsenite on chicken skin fibroblasts.

    Application: WB

    Species: Chicken

    Sample: Chest skin samples (CSFs)

    Antibody concentration: 1/1,000

    Date by conrtesy of: Mrs. Mei Jin, Liaoning Provincial Key Laboratory of Biotechnology and Drug Discovery, Liaoning Normal University

    Title: MicroRNAs involved in the toxic effects of sodium arsenite on chicken skin fibroblasts.

请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

引文

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