DLK-1 Recombinant Rabbit Monoclonal Antibody [PSH04-76]
概述
产品名称
DLK-1 Recombinant Rabbit Monoclonal Antibody [PSH04-76]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein within human DLK-1 aa 1-327 / 383.
种属反应性
Human, Mouse, Rat
验证应用
WB, IHC-P, IF-Tissue
分子量
Predicted band size: 41 kDa
阳性对照
MCF7 cell lysate, HepG2 cell lysate, SH-SY5Y cell lysate, mouse placenta tissue lysate, rat embryo tissue lysate, human pancreas tissue, mouse brain tissue, rat brain tissue.
偶联
unconjugated
克隆号
PSH04-76
反应性数据
| WB | IHC-P | IF-Tissue | |
|---|---|---|---|
| Human |
|
|
|
| Mouse |
|
|
|
| Rat |
|
|
|
| Cow |
|
产品特性
形态
Liquid
浓度
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
-
1:1,000
-
IHC-P
-
1:1,000
-
IF-Tissue
-
1:200
靶点
功能
Protein delta homolog 1, delta like non-canonical Notch ligand 1, fetal antigen 1 or preadipocyte factor 1 is a protein that in humans is encoded by the DLK1 gene. It is expressed as a transmembrane protein, but a soluble form cleaved off by ADAM17 is active in inhibiting adipogenesis, the differentiation of pre-adipocytes into adipocytes. It is a member of the EGF-like family of homeotic proteins. Part of the Dlk1-DIO3 imprinting control region, this gene is one involved in the epigenetic process that causes a subset of genes to be regulated based on their parental origin. Such imprinted genes are required for the formation of the placenta as well as the development of cellular lineages such as those derived from the mesoderm and ectoderm.
背景文献
1. Grassi ES et al. Emerging Roles of DLK1 in the Stem Cell Niche and Cancer Stemness. J Histochem Cytochem. 2022 Jan
2. Zhai Y et al. DLK1-directed chimeric antigen receptor T-cell therapy for hepatocellular carcinoma. Liver Int. 2022 Nov
亚细胞定位
Membrane, Cytoplasm.
别名
Adipocyte differentiation inhibitor protein antibody
Brevideltin antibody
Delta like 1 homolog antibody
Delta like homolog antibody
Delta like protein antibody
Delta1 antibody
DLK 1 antibody
DLK antibody
DLK-1 antibody
DLK1 antibody
展开图片
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Western blot analysis of DLK-1 on different lysates with Rabbit anti-DLK-1 antibody (HA722180) at 1/1,000 dilution.
Lane 1: MCF7 cell lysate (20 µg/Lane)
Lane 2: HepG2 cell lysate (20 µg/Lane)
Lane 3: SH-SY5Y cell lysate (20 µg/Lane)
Lane 4: Mouse placenta tissue lysate (70℃ heat) (40 µg/Lane)
Lane 5: Rat embryo tissue lysate (40 µg/Lane)
Predicted band size: 41 kDa
Observed band size: 40-50kDa
Exposure time: Lane 1-4: 2 minutes; Lane 5: 27 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722180) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Rabbit anti-DLK-1 antibody (HA722180) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722180) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-DLK-1 antibody (HA722180) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722180) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-DLK-1 antibody (HA722180) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722180) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
引文
-
snRNA-seq of adipose tissues reveals the potential cellular and molecular mechanisms of cold and disease resistance in Mongolian cattle
期刊: BMC Genomics
DOI:
IF: 3.5
应用: IF,IHC-P
反应种属: Cow
发表时间: 2024 Oct
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