SMCHD1 Recombinant Rabbit Monoclonal Antibody [JE34-29]
Catalog# HA722141
SMCHD1 Recombinant Rabbit Monoclonal Antibody [JE34-29]
-
WB
-
IHC-P
-
Human
-
unconjugated
概述
产品名称
SMCHD1 Recombinant Rabbit Monoclonal Antibody [JE34-29]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant fragment within Human SMCHD1 aa 1800-2000.
种属反应性
Human
验证应用
WB, IHC-P
分子量
Predicted band size: 226 kDa
阳性对照
293T cell lysate, HepG2 cell lysate, HeLa cell lysate, U-87 MG cell lysate, U-2 OS cell lysate, human breast cancer tissue, human testis tissue, human tonsil tissue.
偶联
unconjugated
克隆号
JE34-29
反应性数据
| WB | IHC-P | |
|---|---|---|
| Human |
|
|
| Mouse |
|
|
| Rat |
|
产品特性
形态
Liquid
浓度
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
-
1:1,000
-
IHC-P
-
1:200
靶点
功能
Structural Maintenance of Chromosomes flexible Hinge Domain Containing 1 (SMCHD1) is a protein that in humans is encoded by the SMCHD1 gene. Mutations in SMCHD1 are causative for development of facioscapulohumeral muscular dystrophy type 2 (FSHD2) and Bosma arhinia microphthalmia syndrome (BAMS). Without maternal SMCHD1 in the egg cell, children bear with altered skeletal structures.
背景文献
1. Gurzau AD et al. Relating SMCHD1 structure to its function in epigenetic silencing. Biochem Soc Trans. 2020 Aug
2. Gurzau AD et al. SMCHD1\'s ubiquitin-like domain is required for N-terminal dimerization and chromatin localization. Biochem J. 2021 Jul
亚细胞定位
Chromosome.
UNIPROT
别名
BAMS antibody
FSHD2 antibody
KIAA0650 antibody
SMC hinge domain containing 1 antibody
SMC hinge domain containing protein 1 antibody
Smchd1 antibody
SMHD1_HUMAN antibody
Structural maintenance of chromosomes flexible hinge domain containing 1 antibody
Structural maintenance of chromosomes flexible hinge domain-containing protein 1 antibody
图片
-
Western blot analysis of SMCHD1 on different lysates with Rabbit anti-SMCHD1 antibody (HA722141) at 1/1,000 dilution.
Lane 1: 293T cell lysate
Lane 2: HepG2 cell lysate
Lane 3: HeLa cell lysate
Lane 4: U-87 MG cell lysate
Lane 5: U-2 OS cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 226 kDa
Observed band size: 226 kDa
Exposure time: 30 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722141) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-SMCHD1 antibody (HA722141) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722141) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-SMCHD1 antibody (HA722141) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722141) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-SMCHD1 antibody (HA722141) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722141) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
浙公网安备 33019202000643号