MARCO Recombinant Rabbit Monoclonal Antibody [PSH04-31]
概述
产品名称
MARCO Recombinant Rabbit Monoclonal Antibody [PSH04-31]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Synthetic peptide within human MARCO aa 401-450 / 520.
种属反应性
Human, Mouse
验证应用
WB, IHC-P
分子量
Predicted band size: 53 kDa
阳性对照
Human liver tissue lysate, human lung tissue lysate, human liver tissue, human spleen tissue, mouse spleen tissue.
偶联
unconjugated
克隆号
PSH04-31
产品特性
形态
Liquid
浓度
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
-
1:1,000-1:2,000
-
IHC-P
-
1:1,000
靶点
功能
Macrophage receptor with collagenous structure (MARCO) is a protein that in humans is encoded by the MARCO gene. MARCO is a class A scavenger receptor that is found on particular subsets of macrophages. Scavenger receptors are pattern recognition receptors (PRRs) found most commonly on immune cells. Their defining feature is that they bind to polyanions and modified forms of a type of cholesterol called low-density lipoprotein (LDL). MARCO is able to bind and phagocytose these ligands and pathogen-associated molecular patterns (PAMPs), leading to the clearance of pathogens and cell signaling events that lead to inflammation. As part of the innate immune system, MARCO clears, or scavenges, pathogens, which leads to inflammatory responses. The scavenger receptor cysteine-rich (SRCR) domain at the end of the extracellular side of MARCO binds ligands to activate the subsequent immune responses. MARCO expression on macrophages has been associated with tumor development and also with Alzheimer's disease, via decreased responses of cells when ligands bind to MARCO.
背景文献
1. Dong Q et al. MARCO is a potential prognostic and immunotherapy biomarker. Int Immunopharmacol. 2023 Mar
2. Yang YS et al. Superenhancer drives a tumor-specific splicing variant of MARCO to promote triple-negative breast cancer progression. Proc Natl Acad Sci U S A. 2022 Nov
亚细胞定位
Cell membrane.
别名
AI323439 antibody
Ly112 antibody
Macrophage receptor MARCO antibody
Macrophage receptor with collagenous structure antibody
Marco antibody
MARCO_HUMAN antibody
SCARA2 antibody
Scavenger receptor class A member 2 antibody
图片
-
Western blot analysis of MARCO on different lysates with Rabbit anti-MARCO antibody (HA722114) at 1/2,000 dilution.
Lane 1: Human liver tissue lysate
Lane 2: Human lung tissue lysate
Lysates/proteins at 40 µg/Lane.
Predicted band size: 53 kDa
Observed band size: 70 kDa
Exposure time: 3 minutes; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722114) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
☑ Cell treatment (CT)
Western blot analysis of MARCO on different lysates with Rabbit anti-MARCO antibody (HA722114) at 1/1,000 dilution.
Lane 1: THP-1 cell lysate
Lane 2: THP-1 treated with 50ng/mL PMA for 72 hours cell lysate
Lysates/proteins at 30 µg/Lane.
Predicted band size: 53 kDa
Observed band size: 53 kDa
Exposure time: 3 minutes; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722114) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-MARCO antibody (HA722114) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722114) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-MARCO antibody (HA722114) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722114) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Rabbit anti-MARCO antibody (HA722114) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722114) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
引文
-
Spatial Location of SPP1+TAMs and Mutually Exclusive Subsets Based on Single-Cell and Transcriptome Data
期刊: Cancer Science
DOI: 10.1111/cas.70247
IF: 4.3
应用: IHC
反应种属: Human
发表时间: 2025 Nov
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