Alpha 1 Acid Glycoprotein Recombinant Rabbit Monoclonal Antibody [JE31-16]
Catalog# HA721396
Alpha 1 Acid Glycoprotein Recombinant Rabbit Monoclonal Antibody [JE31-16]
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WB
-
IHC-P
-
Human
概述
产品名称
Alpha 1 Acid Glycoprotein Recombinant Rabbit Monoclonal Antibody [JE31-16]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Synthetic peptide within Human Alpha 1 Acid Glycoprotein aa 51-100 / 201.
种属反应性
Human
验证应用
WB, IHC-P
分子量
Predicted band size: 23.5 kDa
阳性对照
Human small intestine tissue lysates, human liver tissue.
偶联
unconjugated
克隆号
JE31-16
RRID
产品特性
形态
Liquid
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
-
1:1,000
-
IHC-P
-
1:200
靶点
功能
Alpha-1-acid glycoprotein 1 is a protein that in humans is encoded by the ORM1 gene. This gene encodes a key acute phase plasma protein. Because of its increase due to acute inflammation, this protein is classified as an acute-phase reactant. The specific function of this protein has not yet been determined; however, it may be involved in aspects of immunosuppression. ORM1 has been shown to interact with Plasminogen activator inhibitor-1.
背景文献
1. Joyce KE et al. Postexercise urinary alpha-1 acid glycoprotein is not dependent on hypoxia. J Appl Physiol (1985). 2022 Jan
2. Durmuş M et al. Does alpha-1-acid glycoprotein determine for infertility in polycystic ovary syndrome? Eur J Obstet Gynecol Reprod Biol. 2022 Jul
亚细胞定位
Secreted.
UNIPROT
别名
A1AG1_HUMAN antibody
AGP 1 antibody
AGP A antibody
AGP antibody
AGP1 antibody
Alpha 1 acid glycoprotein antibody
Alpha-1-acid glycoprotein 1 antibody
alpha-1-AGP antibody
Epididymis secretory sperm binding protein Li 153w antibody
glycoprotein, alpha-1-acid, of serum antibody
展开图片
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Western blot analysis of Alpha 1 Acid Glycoprotein on human small intestine tissue lysates with Rabbit anti-Alpha 1 Acid Glycoprotein antibody (HA721396) at 1/1,000 dilution.
Lysates/proteins at 30 µg/Lane.
Predicted band size: 23.5 kDa
Observed band size: 45 kDa (Glycosylation)
Exposure time: 5 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721396) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Alpha 1 Acid Glycoprotein antibody (HA721396) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721396) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"