LAG-3 Recombinant Rabbit Monoclonal Antibody [PSH0-43]
概述
产品名称
LAG-3 Recombinant Rabbit Monoclonal Antibody [PSH0-43]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein within Human LAG-3 aa 1-500 / 525.
种属反应性
Human, Mouse
验证应用
WB, IHC-P
分子量
Predicted band size: 57.4 kDa
阳性对照
NIH/3T3 transfected with LAG3 cell lysate, human tonsil tissue.
偶联
unconjugated
克隆号
PSH0-43
RRID
反应性数据
| WB | IHC-P | |
|---|---|---|
| Human |
|
|
| Mouse |
|
产品特性
形态
Liquid
浓度
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
-
1:2,000
-
IHC-P
-
1:200
靶点
功能
Lymphocyte-activation gene 3, also known as LAG-3, is a protein which in humans is encoded by the LAG3 gene. LAG3, which was discovered in 1990 and was designated CD223 (cluster of differentiation 223) after the Seventh Human Leucocyte Differentiation Antigen Workshop in 2000, is a cell surface molecule with diverse biologic effects on T cell function. It is an immune checkpoint receptor and as such is the target of various drug development programs by pharmaceutical companies seeking to develop new treatments for cancer and autoimmune disorders. In soluble form it is also being developed as a cancer drug in its own right. LAG3's main ligand is MHC class II, to which it binds with higher affinity than CD4. The protein negatively regulates cellular proliferation, activation, and homeostasis of T cells, in a similar fashion to CTLA-4 and PD-1 and has been reported to play a role in Treg suppressive function. Fibrinogen-like protein1 FGL1, a liver-secreted protein, is another (major) LAG3 functional ligand independent of MHC-II. LAG3 also helps maintain CD8+ T cells in a tolerogenic state and, working with PD-1, helps maintain CD8 exhaustion during chronic viral infection. LAG3 is known to be involved in the maturation and activation of dendritic cells.
背景文献
1. Chocarro L et al. Understanding LAG-3 Signaling. Int J Mol Sci. 2021 May
2. Maruhashi T et al. LAG-3: from molecular functions to clinical applications. J Immunother Cancer. 2020 Sep
亚细胞定位
Cell membrane; Secreted.
别名
CD223 antibody
CD223 antigen antibody
FDC protein antibody
LAG-3 antibody
Lag3 antibody
LAG3_HUMAN antibody
Lymphocyte activating 3 antibody
Lymphocyte activation gene 3 antibody
Lymphocyte activation gene 3 protein antibody
Protein FDC antibody
图片
-
☑ Cell treatment (CT)
Western blot analysis of LAG-3 on different lysates with Rabbit anti-LAG-3 antibody (HA721345) at 1/2,000 dilution.
Lane 1: NIH/3T3 transfected with LAG3 expression vector, whole cell lysate
Lane 2: NIH/3T3 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 57.4 kDa
Observed band size: 55/70 kDa
Exposure time: 1 minute 10 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721345) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-LAG-3 antibody (HA721345) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721345) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
引文
-
Combination of LowDose Epigenetic Modifiers and TIC10 for the Activation of Antitumor Immunity and Inhibition of Tumor Growth in Gastrointestinal Cancer
期刊: Cancer Medicine
DOI: 10.1002/cam4.71061
IF: 3.1
应用: IHC
反应种属: Mouse
发表时间: 2025 Jul
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