Menin Recombinant Rabbit Monoclonal Antibody [JE30-97]
Catalog# HA721313
Menin Recombinant Rabbit Monoclonal Antibody [JE30-97]
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WB
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IHC-P
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Human
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unconjugated
概述
产品名称
Menin Recombinant Rabbit Monoclonal Antibody [JE30-97]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Synthetic peptide within Human Menin aa 566-615 / 615.
种属反应性
Human
验证应用
WB, IHC-P
分子量
Predicted band size: 68 kDa
阳性对照
293 cell lysate, PC-3M cell lysate, Jurkat cell lysate, human lung cancer tissue, human testis tissue.
偶联
unconjugated
克隆号
JE30-97
RRID
产品特性
形态
Liquid
浓度
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
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WB
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1:500-1:1,000
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IHC-P
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1:200
靶点
功能
Essential component of a MLL/SET1 histone methyltransferase (HMT) complex, a complex that specifically methylates 'Lys-4' of histone H3 (H3K4). Functions as a transcriptional regulator. Binds to the TERT promoter and represses telomerase expression. Plays a role in TGFB1-mediated inhibition of cell-proliferation, possibly regulating SMAD3 transcriptional activity. Represses JUND-mediated transcriptional activation on AP1 sites, as well as that mediated by NFKB subunit RELA. Positively regulates HOXC8 and HOXC6 gene expression. May be involved in normal hematopoiesis through the activation of HOXA9 expression. Component of the MLL-HCF complex, at least composed of KMT2A/MLL1, MEN1, ASH2L, RBBP5, DPY30, WDR5, HCFC1 and HCFC2. Component of the menin-associated histone methyltransferase complex, at least composed of KMT2B/MLL4, MEN1, ASH2L, RBBP5, DPY30 and WDR5. Interacts with POLR2B. Interacts with POLR2A phosphorylated at 'Ser-5', but not with the unphosphorylated, nor 'Ser-2' phosphorylated POLR2A forms. Interacts with FANCD2 and DBF4. Interacts with JUND (via MBM motif); inhibits the interaction of JUND with MAPK10 and the phosphorylation of JUND by MAP kinases MAPK8 and MAPK10.
背景文献
1. Huang J., Gurung B., Wan B., Matkar S., Veniaminova N.A., Wan K., Merchant J.L., Hua X., Lei M. The same pocket in menin binds both MLL and JUND but has opposite effects on transcription. Nature 482:542-546 (2012).
2. Hughes C.M., Rozenblatt-Rosen O., Milne T.A., Copeland T.D., Levine S.S., Lee J.C., Hayes D.N., Shanmugam K.S., Bhattacharjee A., Meyerson M. Menin associates with a trithorax family histone methyltransferase complex and with the hoxc8 locus. Mol. Cell 13:587-597 (2004).
亚细胞定位
Nucleus
UNIPROT
别名
MEA 1 antibody
MEA1 antibody
MEN 1 antibody
Men1 antibody
MEN1_HUMAN antibody
Menin antibody
Multiple Endocrine Adenomatosis 1 antibody
Multiple Endocrine Neoplasia 1 antibody
SCG 2 antibody
SCG2 antibody
展开图片
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☑ Knockdown (KD)
Western blot analysis of Menin on different lysates with Rabbit anti-Menin antibody (HA721313) at 1/500 dilution.
Lane 1: HCT 116-si NT cell lysate
Lane 2: HCT 116-si Menin cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 68 kDa
Observed band size: 68 kDa
Exposure time: 30 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721313) at 1/500 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of Menin on different lysates with Rabbit anti-Menin antibody (HA721313) at 1/500 dilution.
Lane 1: 293 cell lysate
Lane 2: PC-3M cell lysate
Lane 3: Jurkat cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 68 kDa
Observed band size: 68 kDa
Exposure time: 2 minutes;
8% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721313) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue with Rabbit anti-Menin antibody (HA721313) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721313) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-Menin antibody (HA721313) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721313) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
引文
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Identification of a metabolic-immune signature associated with prognosis in colon cancer and exploration of potential predictive efficacy of immunotherapy response
期刊: Clinical And Experimental Medicine
DOI:
IF: 3.2
应用: WB,IHC-P
反应种属: Human
发表时间: 2025 Jan
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Identification of a metabolic-immune signature associated with prognosis in colon cancer and exploration of potential predictive efficacy of immunotherapy response
期刊: CLINICAL AND EXPERIMENTAL MEDICINE
DOI: 10.1007/s10238-025-01566-6
IF: 3.5
应用: WB,IHC
反应种属: Human
发表时间: 2025 Jan
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