Human IL-17A Recombinant Rabbit Monoclonal Antibody [PS01-20] - BSA and Azide free (Capture)
概述
产品名称
Human IL-17A Recombinant Rabbit Monoclonal Antibody [PS01-20] - BSA and Azide free (Capture)
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant full length protein.
种属反应性
Human, Dog, Cynomolgus monkey
验证应用
ELISA(Cap)
分子量
Predicted band size: 17.5 kDa
阳性对照
Recombinant IL-17A protein.
偶联
unconjugated
克隆号
PS01-20
RRID
产品特性
形态
Liquid
浓度
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
PBS (pH7.4).
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
ELISA(Cap)
-
Use at an assay dependent concentration.
靶点
功能
Effector cytokine of innate and adaptive immune system involved in antimicrobial host defense and maintenance of tissue integrity. Plays an important role in connecting T cell-mediated adaptive immunity and acute inflammatory response to destroy extracellular bacteria and fungi. As a signature effector cytokine of T-helper 17 cells (Th17), primarily induces neutrophil activation and recruitment at infection and inflammatory sites. In airway epithelium, mediates neutrophil chemotaxis via induction of CXCL1 and CXCL5 chemokines. In secondary lymphoid organs, contributes to germinal center formation by regulating the chemotactic response of B cells to CXCL12 and CXCL13, enhancing retention of B cells within the germinal centers, B cell somatic hypermutation rate and selection toward plasma cells. Effector cytokine of a subset of gamma-delta T cells that functions as part of an inflammatory circuit downstream IL1B, TLR2 and IL23A-IL12B to promote neutrophil recruitment for efficient bacterial clearance. Effector cytokine of innate immune cells including invariant natural killer cell (iNKT) and group 3 innate lymphoid cells that mediate initial neutrophilic inflammation. Involved in the maintenance of the integrity of epithelial barriers during homeostasis and pathogen infection.
背景文献
1. Boisson B., Wang C., Pedergnana V., Wu L., Cypowyj S., Rybojad M., Belkadi A., Picard C., Abel L., Fieschi C., Puel A., Li X., Casanova J.L. An ACT1 mutation selectively abolishes interleukin-17 responses in humans with chronic mucocutaneous candidiasis. Immunity 39:676-686 (2013)
2. Liu C., Qian W., Qian Y., Giltiay N.V., Lu Y., Swaidani S., Misra S., Deng L., Chen Z.J., Li X. Act1, a U-box E3 ubiquitin ligase for IL-17 signaling. Sci. Signal. 2:ra63-ra63 (2009)
亚细胞定位
Secreted.
UNIPROT
别名
CTLA 8 antibody
CTLA-8 antibody
CTLA8 antibody
cytotoxic T lymphocyte associated antigen 8 antibody
Cytotoxic T lymphocyte associated protein 8 antibody
Cytotoxic T lymphocyte associated serine esterase 8 antibody
Cytotoxic T-lymphocyte-associated antigen 8 antibody
IL 17 antibody
IL 17A antibody
IL-17 antibody
展开图片
-
![Sandwich ELISA analysis of IL-17A matched pair antibodies<br />Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody <a href="/products/HA721232" style="font-weight: bold;text-decoration: underline;">HA721232</a> [PS01-20] diluted in carbonate/bicarbonate buffer, at a concentration of 4 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 1% BSA/PBST blocking buffer, and incubated with serial diluted Recombinant IL-17A protein (<a href="/products/HA210731" style="font-weight: bold;text-decoration: underline;">HA210731</a>) starting from 2,000 pg/ml to 30.5 pg/ml for 1 hour at 37℃. The plate was washed and incubated with 50 µl per well of detect antibody <a href="/products/HA721233" style="font-weight: bold;text-decoration: underline;">HA721233</a> [PS01-24] (Biotin, 1/2,000) for 1 hour at 37℃. Then the plate was washed and incubated with 50 µl per well of Streptavidin-HRP for 0.5 hour at 37℃. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.<br /><br />Capture/detect antibodies, Recombinant IL-17A protein, Streptavidin-HRP are diluted in 1%BSA/PBST.](https://storage.huabio.cn/huabio/productImg/HA721232_1.jpg?v=20260320230628)
Sandwich ELISA analysis of IL-17A matched pair antibodies
Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody HA721232 [PS01-20] diluted in carbonate/bicarbonate buffer, at a concentration of 4 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 1% BSA/PBST blocking buffer, and incubated with serial diluted Recombinant IL-17A protein (HA210731) starting from 2,000 pg/ml to 30.5 pg/ml for 1 hour at 37℃. The plate was washed and incubated with 50 µl per well of detect antibody HA721233 [PS01-24] (Biotin, 1/2,000) for 1 hour at 37℃. Then the plate was washed and incubated with 50 µl per well of Streptavidin-HRP for 0.5 hour at 37℃. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Capture/detect antibodies, Recombinant IL-17A protein, Streptavidin-HRP are diluted in 1%BSA/PBST.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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