TdT Recombinant Rabbit Monoclonal Antibody [PD01-00]
Catalog# HA721211
TdT Recombinant Rabbit Monoclonal Antibody [PD01-00]
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WB
-
IHC-P
-
Human
-
unconjugated
概述
产品名称
TdT Recombinant Rabbit Monoclonal Antibody [PD01-00]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein within Human TdT aa 1-509.
种属反应性
Human
验证应用
WB, IHC-P
分子量
Predicted band size: 59 kDa
阳性对照
Jurkat cell lysate, MOLT-4 cell lysate, human thymus tissue.
偶联
unconjugated
克隆号
PD01-00
RRID
产品特性
形态
Liquid
浓度
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
-
1:1,000
-
IHC-P
-
1:200
靶点
功能
Terminal deoxynucleotidyl transferase (TdT) is a unique DNA polymerase that changes the addition of deoxynucleoside 5'-triphosphate to the 3'-end of a DNA initiator without template direction. TdT contributes to the generation of junctional diversity in antigen receptors of immature lymphocytes. TdT is expressed in lymphoid precursors of B- and T-cell lineage in thymus and bone marrow. Foci of TdT positive cells maybe observed in peripheral lymphoid tissues. TdT is also present in malignant tumors of lymphoblastic lineage and thymoma. It is a sensitive and specific marker for lymphoblastic lymphoma/leukemia. Anti-TdT is a useful marker for lymphoblastic lymphomas and has been observed in some cases of acute myeloid leukemia.
背景文献
1. Musallam KM et al. Revisiting the non-transfusion-dependent (NTDT) vs. transfusion-dependent (TDT) thalassemia classification 10 years later. Am J Hematol. 2021 Feb
2. Ali M et al. T cells targeted to TdT kill leukemic lymphoblasts while sparing normal lymphocytes. Nat Biotechnol. 2022 Apr
亚细胞定位
Nucleus.
UNIPROT
别名
Deoxynucleotidyltransferase terminal antibody
DNA nucleotidylexotransferase antibody
DNTT antibody
Nucleosidetriphosphate DNA deoxynucleotidylexotransferase antibody
TDT antibody
TDT_HUMAN antibody
Terminal addition enzyme antibody
Terminal deoxynucleotidyltransferase antibody
Terminal deoxyribonucleotidyltransferase antibody
Terminal transferase antibody
图片
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☑ Relative expression (RE)
Western blot analysis of TdT on different lysates with Rabbit anti-TdT antibody (HA721211) at 1/1,000 dilution.
Lane 1: Jurkat cell lysate (20 µg/Lane)
Lane 2: A431 cell lysate (negative) (20 µg/Lane)
Lane 3: Raji cell lysate (negative) (20 µg/Lane)
Lane 4: Daudi cell lysate (negative) (20 µg/Lane)
Lane 5: MOLT-4 cell lysate (20 µg/Lane)
Predicted band size: 59 kDa
Observed band size: 55 kDa
Exposure time: 1 minute; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721211) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human thymus tissue with Rabbit anti-TdT antibody (HA721211) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721211) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
☑ Relative expression (RE)
Immunohistochemical analysis of paraffin-embedded human liver tissue (negative) with Rabbit anti-TdT antibody (HA721211) at 1/50 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721211) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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