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USP24 Recombinant Rabbit Monoclonal Antibody [JE56-50]

RMB: 900 特惠 1500

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Catalog# HA720022

USP24 Recombinant Rabbit Monoclonal Antibody [JE56-50]

Application

  • WB

  • IF-Cell

Reactivity

  • Human

  • Mouse

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

概述

产品名称

USP24 Recombinant Rabbit Monoclonal Antibody [JE56-50]

抗体类型

Recombinant Rabbit monoclonal Antibody

免疫原

Synthetic peptide within C-terminal Human USP24.

种属反应性

Human, Mouse

验证应用

WB, IF-Cell

分子量

Predicted band size: 294 kDa

阳性对照

A549-parental cell lysate, A549-商品名 KD cell lysate, HepG2 cell lysate, HeLa cell lysate, 293T cell lysate, Jurkat cell lysate, NIH/3T3 cell lysate, Mouse lung tissue lysate, HepG2.

偶联

unconjugated

克隆号

JE56-50

RRID

AB_3071962

产品特性

形态

Liquid

浓度

存放说明

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

存储缓冲液

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

亚型

IgG

纯化方式

Protein A affinity purified.

应用稀释度

  • WB

  • 1:2,000

  • IF-Cell

  • 1:50-1:100

靶点

功能

Ubiquitin-specific protease that regulates cell survival in various contexts through modulating the protein stability of some of its substrates including DDB2, MCL1 or TP53. Plays a positive role on ferritinophagy where ferritin is degraded in lysosomes and releases free iron. Modification of cellular proteins by ubiquitin is an essential regulatory mechanism controlled by the coordinated action of multiple ubiquitin-conjugating and deubiquitinating enzymes. USP24 belongs to a large family of cysteine proteases that function as deubiquitinating enzymes .

背景文献

1. Thayer JA. et. al. The PARK10 gene USP24 is a negative regulator of autophagy and ULK1 protein stability. Autophagy. 2020 Jan

2. Wang YC. et. al. USP24 induces IL-6 in tumor-associated microenvironment by stabilizing p300 and β-TrCP and promotes cancer malignancy. Nat Commun. 2018 Sep

亚细胞定位

Cytosol, nucleoplasm, nucleus.

UNIPROT

SwissProt: Q9UPU5 Human

SwissProt: B1AY13 Mouse

别名

Deubiquitinating enzyme 24 antibody

KIAA1057 antibody

Ubiquitin carboxyl terminal hydrolase 24 antibody

Ubiquitin carboxyl-terminal hydrolase 24 antibody

Ubiquitin specific peptidase 24 antibody

Ubiquitin specific processing protease 24 antibody

Ubiquitin specific protease 24 antibody

Ubiquitin thioesterase 24 antibody

Ubiquitin thiolesterase 24 antibody

Ubiquitin-specific-processing protease 24 antibody

展开

图片

  • <span style="font-weight: bold;">☑ Knockdown (KD)</span><br /><br />Western blot analysis of USP24 on different lysates with Rabbit anti-USP24 antibody (<a href="/products/HA720022" style="font-weight: bold;text-decoration: underline;">HA720022</a>) at 1/1,000 dilution.<br /><br />Lane 1: A549-parental cell lysate<br />Lane 2: A549-USP24 KD cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 294 kDa<br />Observed band size: 294 kDa<br /><br />Exposure time: 30 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA720022" style="font-weight: bold;text-decoration: underline;">HA720022</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Knockdown (KD)

    Western blot analysis of USP24 on different lysates with Rabbit anti-USP24 antibody (HA720022) at 1/1,000 dilution.

    Lane 1: A549-parental cell lysate
    Lane 2: A549-USP24 KD cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 294 kDa
    Observed band size: 294 kDa

    Exposure time: 30 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720022) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of USP24 on different lysates with Rabbit anti-USP24 antibody (<a href="/products/HA720022" style="font-weight: bold;text-decoration: underline;">HA720022</a>) at 1/2,000 dilution.<br /><br />Lane 1: HepG2 cell lysate<br />Lane 2: HeLa cell lysate<br />Lane 3: 293T cell lysate<br />Lane 4: Jurkat cell lysate<br />Lane 5: NIH/3T3 cell lysate<br />Lane 6: Mouse lung tissue lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 294 kDa<br />Observed band size: 294 kDa<br /><br />Exposure time: 30 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA720022" style="font-weight: bold;text-decoration: underline;">HA720022</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of USP24 on different lysates with Rabbit anti-USP24 antibody (HA720022) at 1/2,000 dilution.

    Lane 1: HepG2 cell lysate
    Lane 2: HeLa cell lysate
    Lane 3: 293T cell lysate
    Lane 4: Jurkat cell lysate
    Lane 5: NIH/3T3 cell lysate
    Lane 6: Mouse lung tissue lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 294 kDa
    Observed band size: 294 kDa

    Exposure time: 30 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720022) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • ICC staining of USP24 in HepG2 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (<a href="/products/HA720022" style="font-weight: bold;text-decoration: underline;">HA720022</a>, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor&reg;488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

    ICC staining of USP24 in HepG2 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (HA720022, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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