Myelin Basic Protein Recombinant Antibody [JF0943] - Chicken IgY (Chimeric) - BSA and Azide free
Rmb: 5600
Catalog# HA610392
Myelin Basic Protein Recombinant Antibody [JF0943] - Chicken IgY (Chimeric) - BSA and Azide free
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IHC-Fr
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IF-Tissue
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Human
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Mouse
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Rat
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HA601580
含抗保成分
-
unconjugated
概述
产品名称
Myelin Basic Protein Recombinant Antibody [JF0943] - Chicken IgY (Chimeric) - BSA and Azide free
抗体类型
Recombinant Chimeric Antibody
免疫原
Recombinant protein within Human Myelin Basic Protein aa 121-304 / 304.
种属反应性
Human, Mouse, Rat
验证应用
IHC-Fr, IF-Tissue
靶点分子量
Predicted band size: 33 kDa
阳性对照
Human brain tissue, mouse brain tissue, rat brain tissue,
偶联
unconjugated
克隆号
JF0943
产品特性
形态
Liquid
浓度
存放说明
Store at 2-8℃. Avoid freeze.
存储缓冲液
1*PBS (pH7.4).
纯化方式
Protein A affinity purified.
应用稀释度
靶点
功能
Myelin basic protein (MBP) is the major extrinsic membrane protein of central nervous system myelin. MBP phosphorylation at Threonine 125 is a complex regulatory process that modulates the contribution of MBP to the stability of the myelin sheath. Mitogen-activated protein kinases modulate MBP phosphorylation during myelinogenesis and in the demyelinating disease multiple sclerosis. MBP phosphorylation is regulated by high-frequency stimulation but not low-frequency stimulation of the alveus, the myelinated output fibers of the hippocampus. It is proposed that during periods of increased neuronal activity, calcium activates axonal nitric oxide synthase, which generates the intercellular messengers nitric oxide and superoxide and regulates the phosphorylation state of MBP by MAPK.
背景文献
1. Martinsen V et al. Multiple sclerosis and myelin basic protein: insights into protein disorder and disease. Amino Acids. 2022 Jan
2. Bagheri H et al. Myelin basic protein mRNA levels affect myelin sheath dimensions, architecture, plasticity, and density of resident glial cells. Glia. 2024 Oct
亚细胞定位
Myelin membrane, Nucleus.
别名
GDB antibody
Golli MBP antibody
Golli MBP
myelin basic protein antibody
Hemopoietic MBP antibody
HMBPR antibody
HUGO antibody
MBP antibody
MBP_CAVPO antibody
MBP_HUMAN antibody
展开图片
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This data was developed using HA601580, the same antibody clone in a different buffer formulation.
Application: Immunofluorescence (IHC-Fr)
Species: Mouse
Tissue: Cerebral cortex
Sample: Frozen section
Antigen retrieval: Not required
Wash buffer: 1× PBST
Blocking: 10% normal goat serum + 0.5 % Triton X-100 + 0.3 M Glycine in PBS, 10 minutes at room temperature.
Primary antibody: HA601580, 1/200, overnight at 4℃.
Secondary antibody: Goat Anti-Chicken IgY (iFluor™ 488, HA1139), 1.5 hours at room temperature. -
This data was developed using HA601580, the same antibody clone in a different buffer formulation.
Application: Immunofluorescence (IHC-Fr)
Species: Mouse
Tissue: Hippocampus
Sample: Frozen section
Antigen retrieval: Not required
Wash buffer: 1× PBST
Blocking: 10% normal goat serum + 0.5 % Triton X-100 + 0.3 M Glycine in PBS, 10 minutes at room temperature.
Primary antibody: HA601580, 1/200, overnight at 4℃.
Secondary antibody: Goat Anti-Chicken IgY (iFluor™ 488, HA1139), 1.5 hours at room temperature. -
This data was developed using HA601580, the same antibody clone in a different buffer formulation.
Application: Immunofluorescence (IF-tissue)
Species: Human
Tissue: Brain
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃.
Wash buffer: 1× PBST
Endogenous peroxidase blocking: 3% H2O2, 10 minutes.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA601580, 1/500, overnight at 4℃.
Secondary antibody: Goat Anti-Chicken IgY (iFluor™ 488, HA1139), 1.5 hours at room temperature. -
This data was developed using HA601580, the same antibody clone in a different buffer formulation.
Application: Immunofluorescence (IF-tissue)
Species: Mouse
Tissue: Cerebral cortex
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃.
Wash buffer: 1× PBST
Endogenous peroxidase blocking: 3% H2O2, 10 minutes.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA601580, 1/500, overnight at 4℃.
Secondary antibody: Goat Anti-Chicken IgY (iFluor™ 488, HA1139), 1.5 hours at room temperature. -
This data was developed using HA601580, the same antibody clone in a different buffer formulation.
Application: Immunofluorescence (IF-tissue)
Species: Rat
Tissue: Cerebral cortex
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃.
Wash buffer: 1× PBST
Endogenous peroxidase blocking: 3% H2O2, 10 minutes.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA601580, 1/500, overnight at 4℃.
Secondary antibody: Goat Anti-Chicken IgY (iFluor™ 488, HA1139), 1.5 hours at room temperature.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
同靶点 & 同通路的产品
Myelin Basic Protein Recombinant Antibody [JF0943] - Chicken IgY (Chimeric)
Application: IHC-Fr,IF-Tissue
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
Myelin Basic Protein Recombinant Rabbit Monoclonal Antibody [JF0943] - BSA and Azide free
Application: WB,IHC-P,IF-Tissue,IHC-Fr
Reactivity: Human,Mouse,Rat,Cynomolgus monkey,Pig
Conjugate: unconjugated
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