F4/80 Recombinant Antibody [PSH01-87] - Mouse IgG1 (Chimeric) - BSA and Azide free
Catalog# HA610356
F4/80 Recombinant Antibody [PSH01-87] - Mouse IgG1 (Chimeric) - BSA and Azide free
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WB
-
IHC-P
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Mouse
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HA601537
含抗保成分
-
unconjugated
概述
产品名称
F4/80 Recombinant Antibody [PSH01-87] - Mouse IgG1 (Chimeric) - BSA and Azide free
抗体类型
Recombinant Chimeric Antibody
免疫原
Recombinant protein within mouse F4/80 aa 1-650 / 931.
种属反应性
Mouse
验证应用
WB, IHC-P
分子量
Predicted band size: 102 kDa
阳性对照
RAW264.7 cell lysate, Mouse spleen tissue lysate, mouse liver tissue, mouse lung tissue.
偶联
unconjugated
克隆号
PSH01-87
产品特性
形态
Liquid
浓度
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
1*PBS (pH7.4).
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
-
1:5,000
-
IHC-P
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1:2,000-1:5,000
靶点
功能
EGF-like module-containing mucin-like hormone receptor-like 1 also known as F4/80 is a protein encoded by the ADGRE1 gene. EMR1 is a member of the adhesion GPCR family. Adhesion GPCRs are characterized by an extended extracellular region often possessing N-terminal protein modules that is linked to a TM7 region via a domain known as the GPCR-Autoproteolysis INducing (GAIN) domain. EMR1 expression in human is restricted to eosinophils and is a specific marker for these cells. The murine homolog of EMR1, F4/80, is a well-known and widely used marker of murine macrophage populations. The N-terminal fragment (NTF) of EMR1 contains 4-6 Epidermal Growth Factor-like (EGF-like) domains in human and 4-7 EGF-like domains in the mouse. Utilizing F4/80 knockout mice, Lin et al. showed that F4/80 is not necessary for the development of tissue macrophages but is required for the induction of efferent CD8+ regulatory T cells needed for peripheral tolerance.
背景文献
1. Shin AE et al. F4/80(+)Ly6C(high) Macrophages Lead to Cell Plasticity and Cancer Initiation in Colitis. Gastroenterology. 2023 Apr
2. Deng R et al. Periosteal CD68(+) F4/80(+) Macrophages Are Mechanosensitive for Cortical Bone Formation by Secretion and Activation of TGF-beta1. Adv Sci (Weinh). 2022 Jan
亚细胞定位
Cell membrane.
UNIPROT
别名
ADGRE1 antibody
Adhesion G protein coupled receptor E1 antibody
Adhesion G protein-coupled receptor E1 antibody
AGRE1_HUMAN antibody
Cell surface glycoprotein EMR1 antibody
Cell surface glycoprotein F4/80 antibody
DD7A5 7 antibody
Egf like module containing mucin like hormone receptor like 1 antibody
Egf like module containing mucin like hormone receptor like sequence 1 antibody
EGF like module receptor 1 antibody
展开图片
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☑ Relative expression (RE)
Western blot analysis of F4/80 on different lysates with Mouse anti-F4/80 antibody (HA610356) at 1/5,000 dilution.
Lane 1: RAW264.7 cell lysate (no heat) (10 µg/Lane)
Lane 2: L-929 cell lysate (negative) (10 µg/Lane)
Lane 3: Mouse spleen tissue lysate (no heat) (10 µg/Lane)
Notice: no heat means the lysate is not boiled.
Predicted band size: 102 kDa
Observed band size: 150 kDa
Exposure time: 1 minute 2 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610356) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Mouse anti-F4/80 antibody (HA610356) at 1/5,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610356) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse lung tissue with Mouse anti-F4/80 antibody (HA610356) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610356) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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