VGLUT2 Recombinant Antibody [PSH10-54] - Mouse IgG1 (Chimeric) - BSA and Azide free
Catalog# HA610285
VGLUT2 Recombinant Antibody [PSH10-54] - Mouse IgG1 (Chimeric) - BSA and Azide free
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IHC-Fr
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IHC-P
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WB
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Mouse
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Rat
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HA601451
含抗保成分
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unconjugated
概述
产品名称
VGLUT2 Recombinant Antibody [PSH10-54] - Mouse IgG1 (Chimeric) - BSA and Azide free
抗体类型
Recombinant Chimeric Antibody
种属反应性
Mouse, Rat
验证应用
IHC-Fr, IHC-P, WB
分子量
Predicted band size: 65 kDa
阳性对照
Mouse cerebellum tissue, rat cerebellum tissue, Mouse brain tissue lysate.
偶联
unconjugated
克隆号
PSH10-54
产品特性
形态
Liquid
浓度
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
PBS (pH7.4).
纯化方式
Protein A affinity purified.
应用稀释度
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IHC-Fr
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1:500
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IHC-P
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1:2,000
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WB
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1:5,000
靶点
功能
Predicted to enable L-glutamate transmembrane transporter activity and neurotransmitter transmembrane transporter activity. Involved in neurotransmitter loading into synaptic vesicle. Predicted to be located in synaptic vesicle. Predicted to be active in excitatory synapse. Predicted to be integral component of synaptic vesicle membrane.
背景文献
1. Buck SA et al. Roles of VGLUT2 and Dopamine/Glutamate Co-Transmission in Selective Vulnerability to Dopamine Neurodegeneration. ACS Chem Neurosci. 2022 Jan
2. Zhang Y et al. VGluT2 neuron subtypes in the paraventricular thalamic nucleus regulate depression in paraquat-induced Parkinson\'s disease. J Hazard Mater. 2024 Jul
亚细胞定位
Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane, Synapse, synaptosome, Cell membrane.
别名
Differentiation associated BNPI antibody
Differentiation associated Na dependent inorganic phosphate cotransporter antibody
Differentiation associated Na(+) dependent inorganic phosphate cotransporter antibody
Differentiation-associated BNPI antibody
Differentiation-associated Na(+)-dependent inorganic phosphate cotransporter antibody
DNPI antibody
SLC17A6 antibody
Sodium dependent inorganic phosphate cotransporter antibody
Solute carrier family 17 (Sodium dependent inorganic phosphate cotransporter) member 6 antibody
Solute carrier family 17 member 6 antibody
展开图片
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Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Mouse anti-VGLUT2 antibody (HA610285) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610285) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Mouse anti-VGLUT2 antibody (HA610285) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610285) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Western blot analysis of VGLUT2 on different lysates with Mouse anti-VGLUT2 antibody (HA610285) at 1/5,000 dilution.
Lane 1: Mouse brain tissue lysate
Lane 2: Mouse lung tissue lysate (negative)
Lysates/proteins at 20 µg/Lane.
Predicted band size: 65 kDa
Observed band size: 65 kDa
Exposure time: 20 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610285) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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