Calretinin Recombinant Mouse Monoclonal Antibody [PSH08-29] - BSA and Azide free
Rmb: 5600
Catalog# HA610215
Calretinin Recombinant Mouse Monoclonal Antibody [PSH08-29] - BSA and Azide free
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WB
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IHC-P
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IHC-Fr
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IF-Tissue
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IP
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Mouse
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Rat
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HA601369
含抗保成分
-
unconjugated
概述
产品名称
Calretinin Recombinant Mouse Monoclonal Antibody [PSH08-29] - BSA and Azide free
抗体类型
Recombinant Mouse Monoclonal Antibody
免疫原
Recombinant protein within human Calretinin aa 1-200.
种属反应性
Mouse, Rat
验证应用
WB, IHC-P, IHC-Fr, IF-Tissue, IP
靶点分子量
Predicted band size: 32 kDa
阳性对照
Mouse brain tissue lysate, Mouse colon tissue lysate, Rat brain tissue lysate, Rat colon tissue lysate, mouse brain tissue, rat brain tissue.
偶联
unconjugated
克隆号
PSH08-29
产品特性
形态
Liquid
浓度
存放说明
Store at 2-8℃. Avoid freeze.
存储缓冲液
1*PBS (pH7.4).
亚型
IgG1
纯化方式
Protein A affinity purified.
应用稀释度
靶点
功能
Calretinin, also known as calbindin 2 (formerly 29 kDa calbindin), is a calcium-binding protein involved in calcium signaling. In humans, the calretinin protein is encoded by the CALB2 gene. This gene encodes an intracellular calcium-binding protein belonging to the troponin C superfamily. Members of this protein family have six EF-hand domains which bind calcium. This protein plays a role in diverse cellular functions, including message targeting and intracellular calcium buffering. Calretinin is abundantly expressed in neurons including retina (which gave it the name) and cortical interneurons. Expression was found in different neurons than that of the similar vitamin D-dependent calcium-binding protein, calbindin-28kDa. Calretinin has an important role as a modulator of neuronal excitability including the induction of long-term potentiation. Loss of expression of calretinin in hippocampal interneurons has been suggested to be relevant in temporal lobe epilepsy. It is expressed in a number of other locations including hair follicles.
背景文献
1. Francavilla C et al. Phosphoproteomics of Primary Cells Reveals Druggable Kinase Signatures in Ovarian Cancer. Cell Rep 18:3242-3256 (2017).
2. McMahon SM et al. Multiple cytosolic calcium buffers in posterior pituitary nerve terminals. J Gen Physiol 147:243-54 (2016).
亚细胞定位
Cytosol, dendrite, gap junction, nucleus, parallel fiber to Purkinje cell synapse, synapse, terminal bouton.
别名
29 kDa calbindin antibody
CAB 29 antibody
CAB29 antibody
CAL 2 antibody
CAL2 antibody
CALB 2 antibody
CALB2 antibody
CALB2_HUMAN antibody
Calbindin 2 29kDa antibody
Calbindin 2 antibody
展开图片
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This data was developed using HA601369, the same antibody clone in a different buffer formulation.
Western blot analysis of Calretinin on different lysates with Mouse anti-Calretinin antibody (HA601369) at 1/2,000 dilution.
Lane 1: Mouse brain tissue lysate (20 µg/Lane)
Lane 2: Mouse colon tissue lysate (20 µg/Lane)
Lane 3: Rat brain tissue lysate (20 µg/Lane)
Lane 4: Rat colon tissue lysate (20 µg/Lane)
Predicted band size: 32 kDa
Observed band size: 29 kDa
Exposure time: 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601369) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. -
This data was developed using HA601369, the same antibody clone in a different buffer formulation.
Application: IHC-Fr
Species: Mouse
Site: Hippocampus
Sample: Frozen section
Antibody concentration: 1/500
Antigen retrieval: The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. -
This data was developed using HA601369, the same antibody clone in a different buffer formulation.
Application: IHC-Fr
Species: Mouse
Site: Retina
Sample: Frozen section
Antibody concentration: 1/500
Antigen retrieval: The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. -
This data was developed using HA601369, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Mouse anti-Calretinin antibody (HA601369) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601369) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using HA601369, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Mouse anti-Calretinin antibody (HA601369) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601369) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using HA601369, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Mouse anti-Calretinin antibody (HA601369) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601369) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
This data was developed using HA601369, the same antibody clone in a different buffer formulation.
Application: IF-tissue
Species: Mouse
Site: Hippocampus
Sample: Paraffin-embedded section
Antibody concentration: 1/500 -
This data was developed using HA601369, the same antibody clone in a different buffer formulation.
Calretinin was immunoprecipitated from 0.2 mg mouse brain tissue lysate with HA601369 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA601369 at 1/2,000 dilution. Anti-Mouse IgG for IP, AlpSdAbs® VHH(HRP) (001-100-005) at 1/5,000 dilution was used for 1 hour at room temperature.
Lane 1: Mouse brain tissue lysate (input)
Lane 2: HA601369 IP in mouse brain tissue lysate
Lane 3: Mouse IgG instead of HA601369 in mouse brain tissue lysate
Blocking/Dilution buffer: Primary Antibody Dilution
Exposure time: 40 seconds; ECL: K1801
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