IREB2 Recombinant Mouse Monoclonal Antibody [9D1-R] - BSA and Azide free
Catalog# HA610146
IREB2 Recombinant Mouse Monoclonal Antibody [9D1-R] - BSA and Azide free
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WB
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Human
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Mouse
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Rat
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HA601271
含抗保成分
-
unconjugated
概述
产品名称
IREB2 Recombinant Mouse Monoclonal Antibody [9D1-R] - BSA and Azide free
抗体类型
Recombinant Mouse Monoclonal Antibody
免疫原
Recombinant protein within human IREB2 aa 1-963.
种属反应性
Human, Mouse, Rat
验证应用
WB
分子量
Predicted band size: 105 kDa
阳性对照
Jurkat cell lysate, A549 cell lysate, Mouse liver tissue lysate, Mouse kidney tissue lysate, Mouse heart tissue lysate, Rat liver tissue lysate.
偶联
unconjugated
克隆号
9D1-R
产品特性
形态
Liquid
浓度
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
1*PBS (pH7.4).
亚型
IgG1
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
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1:2,000
靶点
功能
RNA-binding protein that binds to iron-responsive elements (IRES), which are stem-loop structures found in the 5'-UTR of ferritin, and delta aminolevulinic acid synthase mRNAs, and in the 3'-UTR of transferrin receptor mRNA. Binding to the IRE element in ferritin results in the repression of its mRNA translation. Binding of the protein to the transferrin receptor mRNA inhibits the degradation of this otherwise rapidly degraded mRNA.
背景文献
1. Samaniego F. et. al. Molecular characterization of a second iron-responsive element binding protein, iron regulatory protein 2. Structure, function, and post-translational regulation. J. Biol. Chem. 269:30904-30910(1994).
亚细胞定位
Cytoplasm.
别名
ACO3 antibody
FLJ23381 antibody
IRE BP2 antibody
IRE-BP 2 antibody
IREB 2 antibody
IREB2 antibody
IREB2_HUMAN antibody
Iron regulatory protein 2 antibody
iron responsive element binding protein 2 antibody
Iron-responsive element-binding protein 2 antibody
展开图片
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Western blot analysis of IREB2 on different lysates with Mouse anti-IREB2 antibody (HA610146) at 1/2,000 dilution.
Lane 1: Jurkat cell lysate (20 µg/Lane)
Lane 2: A549 cell lysate (20 µg/Lane)
Lane 3: Mouse liver tissue lysate (40 µg/Lane)
Lane 4: Mouse kidney tissue lysate (40 µg/Lane)
Lane 5: Mouse heart tissue lysate (40 µg/Lane)
Lane 6: Rat liver tissue lysate (40 µg/Lane)
Predicted band size: 105 kDa
Observed band size: 105 kDa
Exposure time: 25 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610146) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Anti-Mouse IgG for IP Nano-secondary antibody (NBI02H) at 1/5,000 dilution was used for 1 hour at room temperature.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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