Oct4 Recombinant Mouse Monoclonal Antibody [A3-C11-R] - BSA and Azide free
Catalog# HA610085
Oct4 Recombinant Mouse Monoclonal Antibody [A3-C11-R] - BSA and Azide free
-
WB
-
IF-Cell
-
Human
概述
产品名称
Oct4 Recombinant Mouse Monoclonal Antibody [A3-C11-R] - BSA and Azide free
抗体类型
Recombinant Mouse Monoclonal Antibody
免疫原
Recombinant protein within full length human Oct4 protein.
种属反应性
Human
验证应用
WB, IF-Cell
分子量
Predicted band size: 39 kDa
阳性对照
NCCIT cell lysates, NCCIT.
偶联
unconjugated
克隆号
A3-C11-R
产品特性
形态
Liquid
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
PBS (pH7.4).
亚型
IgG1
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
-
1:1,000
-
IF-Cell
-
1:100
靶点
功能
Oct-4 (POU5F1) is transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). It forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Oct-4 is critical for early embryogenesis and for embryonic stem cell pluripotency. It is expressed in developing brain and highest levels also found in specific cell layers of the cortex, the olfactory bulb, the hippocampus and the cerebellum.
背景文献
1. Oct4 pseudogenes are transcribed in cancers."Suo G., Han J., Wang X., Zhang J., Zhao Y., Zhao Y., Dai J.Biochem. Biophys. Res. Commun. 337:1047-1051(2005)
2. Sumoylation of Oct4 enhances its stability, DNA binding, and transactivation."Wei F., Schoeler H.R., Atchison M.L.J. Biol. Chem. 282:21551-21560(2007)
3. Post-translational modification of POU domain transcription factor Oct-4 by SUMO-1."Zhang Z., Liao B., Xu M., Jin Y.FASEB J. 21:3042-3051(2007)
亚细胞定位
Cytoplasm, Nucleus.
UNIPROT
别名
Octamer binding transcription factor 4 antibody
MGC22487 antibody
Oct 3 antibody
Oct 4 antibody
Oct-3 antibody
Oct-4 antibody
OCT3 antibody
Oct4 antibody
Octamer binding protein 3 antibody
Octamer binding protein 4 antibody
展开图片
-
Western blot analysis of Oct4 on NCCIT cell lysates with Mouse anti-Oct4 antibody (HA610085) at 1/1,000 dilution.
Lysates/proteins at 20 µg/Lane.
Predicted band size: 39 kDa
Observed band size: 45 kDa
Exposure time: 1 minute 59 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610085) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. -
☑ Knockdown (KD)
Western blot analysis of Oct4 on different lysates with Mouse anti-Oct4 antibody (HA610085) at 1/1,000 dilution.
Lane 1: HAP1-parental cell lysate
Lane 2: HAP1-Oct4 KD cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 39 kDa
Observed band size: 42 kDa
Exposure time: 18 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610085) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunocytochemistry analysis of NCCIT cells labeling Oct4 with Mouse anti-Oct4 antibody (HA610085) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-Oct4 antibody (HA610085) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"