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BCL6 Recombinant Mouse Monoclonal Antibody [A6C7-R] - BSA and Azide free

RMB: 5600

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Catalog# HA610020

BCL6 Recombinant Mouse Monoclonal Antibody [A6C7-R] - BSA and Azide free

Application

  • IHC-P

  • IF-Cell

Reactivity

  • Human

With BSA and Azide
Conjugation

  • unconjugated

概述

产品名称

BCL6 Recombinant Mouse Monoclonal Antibody [A6C7-R] - BSA and Azide free

抗体类型

Recombinant Mouse Monoclonal Antibody

免疫原

Recombinant protein within Human BCL-6 aa 301-500 / 706.

种属反应性

Human

验证应用

IHC-P, IF-Cell

分子量

Predicted band size: 79 kDa

阳性对照

Human B lymphocytoma tissue, human lymph nodes tissue, Raji, Human tonsil.

偶联

unconjugated

克隆号

A6C7-R

产品特性

形态

Liquid

存放说明

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

存储缓冲液

PBS (pH7.4).

亚型

IgG1

纯化方式

Protein A affinity purified.

应用稀释度

  • IHC-P

  • 1:1,000

  • IF-Cell

  • 1:200

靶点

功能

The Bcl-6 protein (B-cell CLL/lymphoma 6, zinc finger protein 51) is a nuclear zinc finger transcription factor with an N-terminal POZ domain. Bcl-6 represses p53 transcription by binding 2 specific DNA sites within the p53 promoter region and accordingly, p53 expression is absent in germinal centre B-cells where Bcl-6 is highly expressed. Most notably, constitutive expression of Bcl-6 protects B-cell lines from apoptosis induced by DNA damage. Bcl-6 is mainly used in the classification of B-cell lymphomas. In malignant lymphomas, Bcl-6 protein is most commonly detected in germinal centre neoplasms including follicular lymphoma, Burkitt lymphoma, diffuse large B-cell lymphoma (40%), lymphocyte predominant Hodgkin lymphoma, and even in peripheral T-cell lymphoma with follicular localization (“follicular T cell lymphoma”). It is also found in CD30+ anaplastic large cell lymphoma. Bcl-6 expression is virtually absent in acute lymphatic leukaemia and mantle cell lymphoma. Normal tonsil is recommended as positive and negative tissue control. Virtually all the germinal centre B-cells must show a moderate to strong nuclear staining reaction, while an at least weak to moderate nuclear staining reaction must be seen in the majority of squamous epithelial cells. In the mantle zones and interfollicular areas only dispersed cells should display a positive nuclear staining reaction.

背景文献

1. Guo J. et. al. BCL6 confers KRAS-mutant non-small-cell lung cancer resistance to BET inhibitors. J Clin Invest. 2021 Jan

2. Leeman-Neill RJ. et. al. BCL6 as a therapeutic target for lymphoma. Expert Opin Ther Targets. 2018 Feb

亚细胞定位

Nucleus.

UNIPROT

SwissProt: P41182 Human

别名

B cell CLL/lymphoma 6 antibody

B cell lymphoma 6 protein antibody

B-cell lymphoma 5 protein antibody

B-cell lymphoma 6 protein antibody

BCL 5 antibody

Bcl 6 antibody

BCL-5 antibody

BCL-6 antibody

BCL5 antibody

BCL6 antibody

展开

图片

  • Immunohistochemical analysis of paraffin-embedded human B lymphocytoma tissue with Mouse anti-BCL6 antibody (<a href="/products/HA610020" style="font-weight: bold;text-decoration: underline;">HA610020</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA610020" style="font-weight: bold;text-decoration: underline;">HA610020</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human B lymphocytoma tissue with Mouse anti-BCL6 antibody (HA610020) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610020) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human lymph nodes tissue with Mouse anti-BCL6 antibody (<a href="/products/HA610020" style="font-weight: bold;text-decoration: underline;">HA610020</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA610020" style="font-weight: bold;text-decoration: underline;">HA610020</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human lymph nodes tissue with Mouse anti-BCL6 antibody (HA610020) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610020) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunocytochemistry analysis of Raji cells labeling BCL6 with Mouse anti-BCL6 antibody (<a href="/products/HA610020" style="font-weight: bold;text-decoration: underline;">HA610020</a>) at 1/200 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-BCL6 antibody (<a href="/products/HA610020" style="font-weight: bold;text-decoration: underline;">HA610020</a>) at 1/200 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor&trade; 488, <a href="/products/HA1125" style="font-weight: bold;text-decoration: underline;">HA1125</a>) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

    Immunocytochemistry analysis of Raji cells labeling BCL6 with Mouse anti-BCL6 antibody (HA610020) at 1/200 dilution.

    Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-BCL6 antibody (HA610020) at 1/200 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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