F4/80 Recombinant Antibody [PSH0-85] - Rat IgG1 (Chimeric)
概述
产品名称
F4/80 Recombinant Antibody [PSH0-85] - Rat IgG1 (Chimeric)
抗体类型
Recombinant Chimeric Antibody
免疫原
Recombinant protein within mouse F4/80 aa 1-650 / 931.
种属反应性
Mouse, Rat
验证应用
IHC-P, mIHC
分子量
Predicted band size: 102 kDa
阳性对照
Mouse liver tissue, rat liver tissue.
偶联
unconjugated
克隆号
PSH0-85
反应性数据
| IHC-P | mIHC | |
|---|---|---|
| Mouse |
|
|
| Rat |
|
|
| Human |
|
产品特性
形态
Liquid
浓度
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
纯化方式
Protein A affinity purified.
应用稀释度
-
IHC-P
-
1:1,000
-
mIHC
-
1:200
靶点
功能
EGF-like module-containing mucin-like hormone receptor-like 1 also known as F4/80 is a protein encoded by the ADGRE1 gene. EMR1 is a member of the adhesion GPCR family. Adhesion GPCRs are characterized by an extended extracellular region often possessing N-terminal protein modules that is linked to a TM7 region via a domain known as the GPCR-Autoproteolysis INducing (GAIN) domain. EMR1 expression in human is restricted to eosinophils and is a specific marker for these cells. The murine homolog of EMR1, F4/80, is a well-known and widely used marker of murine macrophage populations. The N-terminal fragment (NTF) of EMR1 contains 4-6 Epidermal Growth Factor-like (EGF-like) domains in human and 4-7 EGF-like domains in the mouse. Utilizing F4/80 knockout mice, Lin et al. showed that F4/80 is not necessary for the development of tissue macrophages but is required for the induction of efferent CD8+ regulatory T cells needed for peripheral tolerance.
背景文献
1. Deng R et al. Periosteal CD68+ F4/80+ Macrophages Are Mechanosensitive for Cortical Bone Formation by Secretion and Activation of TGF-β1. Adv Sci (Weinh). 2022 Jan
2. Shin AE et al. F4/80+Ly6Chigh Macrophages Lead to Cell Plasticity and Cancer Initiation in Colitis. Gastroenterology. 2023 Apr
亚细胞定位
Cell membrane.
别名
ADGRE1 antibody
Adhesion G protein coupled receptor E1 antibody
Adhesion G protein-coupled receptor E1 antibody
AGRE1_HUMAN antibody
Cell surface glycoprotein EMR1 antibody
Cell surface glycoprotein F4/80 antibody
DD7A5 7 antibody
Egf like module containing mucin like hormone receptor like 1 antibody
Egf like module containing mucin like hormone receptor like sequence 1 antibody
EGF like module receptor 1 antibody
展开图片
-
Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rat anti-F4/80 antibody (HA601485) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601485) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat liver tissue with Rat anti-F4/80 antibody (HA601485) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601485) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
引文
-
In silico optimized cell-penetrating peptides achieve transdermal siRNA delivery and regulate inflammatory environment in psoriasis
期刊: Biomaterials
DOI: 10.1016/j.biomaterials.2025.123882
IF: 12.9
应用: mIHC
反应种属: Mouse
发表时间: 2025 Dec
-
High-Fat Diet Promotes Glycolysis in Hepatocellular Carcinoma by Suppressing Hepatic Kisspeptin Signaling in Mice
期刊: Molecular Carcinogenesis
DOI: 10.1002/mc.70068
IF: 3.2
应用: IHC
反应种属: Mouse
发表时间: 2025 Dec
浙公网安备 33019202000643号