Mouse CD45R Recombinant Rat Monoclonal Antibody [PSH10-80]
Catalog# HA601385
Mouse CD45R Recombinant Rat Monoclonal Antibody [PSH10-80]
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IHC-P
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FC
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IF-Tissue
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Mouse
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HA610229
不含抗保成分
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unconjugated
概述
产品名称
Mouse CD45R Recombinant Rat Monoclonal Antibody [PSH10-80]
抗体类型
Recombinant Rat Monoclonal Antibody
种属反应性
Mouse
验证应用
IHC-P, FC, IF-Tissue
分子量
Predicted band size: 145 kDa
阳性对照
Mouse spleen tissue, mouse spleen cells.
偶联
unconjugated
克隆号
PSH10-80
反应性数据
| IHC-P | FC | IF-Tissue | |
|---|---|---|---|
| Mouse |
|
|
|
产品特性
形态
Liquid
浓度
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG1
纯化方式
Protein A affinity purified.
应用稀释度
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IHC-P
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1:1,000
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FC
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1:1,000
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IF-Tissue
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1:500
靶点
功能
Protein tyrosine phosphatase, receptor type, C also known as PTPRC is an enzyme that, in humans, is encoded by the PTPRC gene. PTPRC is also known as CD45 antigen (CD stands for cluster of differentiation), which was originally called leukocyte common antigen (LCA). PTPRC is a critical enzyme involved in regulating immune cell function. PTPRC is a transmembrane protein tyrosine phosphatase expressed on the surface of all nucleated hematopoietic cells, particularly lymphocytes. It plays a key role in the activation and differentiation of T cells, B cells, and other immune cells by modulating signaling pathways. It functions by dephosphorylating specific tyrosine residues on target proteins, thereby controlling various signaling processes essential for immune response and homeostasis.
背景文献
1. Albekairi TH et al. Cadmium exposure exacerbates immunological abnormalities in a BTBR T(+) Itpr3(tf)/J autistic mouse model by upregulating inflammatory mediators in CD45R-expressing cells. J Neuroimmunol. 2024 Jan
2. Farombi EO et al. Kolaviron ameliorates behavioural deficit and injury to striatal dopaminergic terminals via modulation of oxidative burden, DJ-1 depletion and CD45R(+) cells infiltration in MPTP-model of Parkinson\'s disease. Metab Brain Dis. 2020 Aug
亚细胞定位
Cell membrane, Membrane raft, Synapse.
UNIPROT
别名
B220 antibody
CD45 antibody
CD45 antigen antibody
Glycoprotein antibody
GP180 antibody
L CA antibody
L-CA antibody
LCA antibody
Leukocyte common antigen antibody
Leukocyte common antigen precursor antibody
展开图片
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Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Rat anti-Mouse CD45R antibody (HA601385) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601385) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Flow cytometric analysis of mouse spleen cells labeling Mouse CD45R (HA601385).
Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA601385, 1/1,000). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a Dylight 488 conjugate-Goat anti-Rat IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. -
Application: IF-Tissue
Species: Mouse
Site: spleen
Sample: Paraffin-embedded section
Antibody concentration: 1/500
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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