Kindlin 2 Mouse Monoclonal Antibody [A10G12]
Catalog# HA601343
Kindlin 2 Mouse Monoclonal Antibody [A10G12]
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WB
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IF-Cell
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IP
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Human
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Mouse
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Rat
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unconjugated
概述
产品名称
Kindlin 2 Mouse Monoclonal Antibody [A10G12]
抗体类型
Mouse Monoclonal Antibody
免疫原
Synthetic peptide within human Kindlin 2 aa 301-350 / 680.
种属反应性
Human, Mouse, Rat
验证应用
WB, IF-Cell, IP
分子量
Predicted band size: 78 kDa
阳性对照
MDA-MB-231 cell lysate, Saos-2 cell lysate, mouse heart tissue lysate, mouse lung tissue lysate, rat heart tissue lysate, rat lung tissue lysate, MDA-MB-231.
偶联
unconjugated
克隆号
A10G12
反应性数据
| WB | IF-Cell | IP | |
|---|---|---|---|
| Human |
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| Mouse |
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| Rat |
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产品特性
形态
Liquid
浓度
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG2a
纯化方式
Protein A affinity purified.
应用稀释度
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WB
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1:1,000
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IF-Cell
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1:100
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IP
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1-2μg/sample
靶点
功能
Fermitin family homolog 2 (FERMT2) also known as pleckstrin homology domain-containing family C member 1 (PLEKHC1) or kindlin-2 is a protein that in humans is encoded by the FERMT2 gene. Kindlin-2 is the first of the kindlin protein to be discovered in 1994. It was detected in a screen for epidermal growth factor (EGF)-induced mRNAs and initially named mitogen-inducible gene 2 (Mig-2) protein. FERMT2 is a component of extracellular matrix structures in mammalian cells and is required for proper control of cell shape change. A major task of kindlins is to regulate the activation of integrins. FERMT2 has been shown to interact with FBLIM1.
背景文献
1. Gao H et al. Kindlin-2 haploinsufficiency protects against fatty liver by targeting Foxo1 in mice. Nat Commun. 2022 Feb
2. Wu X et al. Kindlin-2 preserves integrity of the articular cartilage to protect against osteoarthritis. Nat Aging. 2022 Apr
亚细胞定位
Cytoplasm, Nucleus, Cell surface.
别名
FLJ34213 antibody
FLJ44462 antibody
UNC112 antibody
FERM2_HUMAN antibody
Fermitin family homolog 2 antibody
Fermt2 antibody
KIND2 antibody
Kindlin-2 antibody
MIG-2 antibody
MIG2 antibody
展开图片
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Western blot analysis of Kindlin 2 on different lysates with Mouse anti-Kindlin 2 antibody (HA601343) at 1/1,000 dilution.
Lane 1: MDA-MB-231 cell lysate (20 µg/Lane)
Lane 2: Saos-2 cell lysate (20 µg/Lane)
Lane 3: Mouse heart tissue lysate (40 µg/Lane)
Lane 4: Mouse lung tissue lysate (40 µg/Lane)
Lane 5: Rat heart tissue lysate (40 µg/Lane)
Lane 6: Rat lung tissue lysate (40 µg/Lane)
Predicted band size: 78 kDa
Observed band size: 72 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601343) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Anti-Mouse IgG for IP Nano-secondary antibody (NBI02H) at 1/5,000 dilution was used for 1 hour at room temperature. -
Immunocytochemistry analysis of MDA-MB-231 cells labeling Kindlin 2 with Mouse anti-Kindlin 2 antibody (HA601343) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-Kindlin 2 antibody (HA601343) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution. -
Kindlin 2 was immunoprecipitated from 0.2 mg MDA-MB-231 cell lysate with HA601343 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA601343 at 1/2,000 dilution. Anti-Mouse IgG for IP Nano-secondary antibody (NBI02H) at 1/5,000 dilution was used for 1 hour at room temperature.
Lane 1: MDA-MB-231 cell lysate (input)
Lane 2: HA601343 IP in MDA-MB-231 cell lysate
Lane 3: Mouse IgG instead of HA601343 in MDA-MB-231 cell lysate
Blocking/Dilution buffer: 5% NFDM/TBST
Exposure time: 38 seconds; ECL: K1801
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
引文
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Kindlin-2 mediates Peyronie's disease through activation of TGF-β/Smad signaling pathway under the presence of TGF-β1
期刊: Cellular Signalling
DOI: 10.1016/j.cellsig.2024.111286
IF: 4.4
应用: IP
反应种属: Rat
发表时间: 2024 Jul
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