NAPSIN A Recombinant Mouse Monoclonal Antibody [A9C1-R]
Catalog# HA601302
NAPSIN A Recombinant Mouse Monoclonal Antibody [A9C1-R]
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IHC-P
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Human
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HA610175
不含抗保成分
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unconjugated
概述
产品名称
NAPSIN A Recombinant Mouse Monoclonal Antibody [A9C1-R]
抗体类型
Recombinant Mouse Monoclonal Antibody
免疫原
Recombinant protein within human NAPSIN A aa 51-200 / 420.
种属反应性
Human
验证应用
IHC-P
分子量
Predicted band size: 45 kDa
阳性对照
Human kidney tissue, human lung cancer tissue.
偶联
unconjugated
克隆号
A9C1-R
RRID
产品特性
形态
Liquid
浓度
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG1
纯化方式
Protein A affinity purified.
应用稀释度
-
IHC-P
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1:2,000
靶点
功能
Napsin-A is an aspartic proteinase that is encoded in humans by the NAPSA gene. The name napsin comes from novel aspartic proteinase of the pepsin family. The activation peptide of an aspartic proteinase acts as an inhibitor of the active site. These peptide segments, or pro-parts, are deemed important for correct folding, targeting, and control of the activation of aspartic proteinase zymogens. The pronapsin A gene is expressed predominantly in lung and kidney. Its translation product is predicted to be a fully functional, glycosylated aspartic proteinase precursor containing an RGD motif and an additional 18 residues at its C-terminus. Detection of NAPSA gene expression can be used to distinguish adenocarcinomas from other forms of lung cancer.
背景文献
1. Weidemann S et al. Napsin A Expression in Human Tumors and Normal Tissues. Pathol Oncol Res. 2021 Apr
2. Wu J et al. Napsin A Expression in Subtypes of Thyroid Tumors: Comparison with Lung Adenocarcinomas. Endocr Pathol. 2020 Mar
亚细胞定位
Secreted.
UNIPROT
别名
Asp 4 antibody
ASP4 antibody
Aspartyl protease 4 antibody
KAP antibody
Kdap antibody
Kidney derived aspartic protease like protein antibody
NAP1 antibody
NAPA antibody
Napsa antibody
NAPSA_HUMAN antibody
展开图片
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Immunohistochemical analysis of paraffin-embedded human kidney tissue with Mouse anti-NAPSIN A antibody (HA601302) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601302) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue with Mouse anti-NAPSIN A antibody (HA601302) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601302) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
☑ Relative expression (RE)
Immunohistochemical analysis of paraffin-embedded human colon tissue (negative) with Mouse anti-NAPSIN A antibody (HA601302) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601302) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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