VP40 Mouse Monoclonal Antibody [B6-A8]
Catalog# HA601066
VP40 Mouse Monoclonal Antibody [B6-A8]
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ELISA
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unconjugated
概述
产品名称
VP40 Mouse Monoclonal Antibody [B6-A8]
抗体类型
Mouse Monoclonal Antibody
免疫原
Synthetic peptide within Zaire ebolavirus VP40 aa 260-300.
验证应用
ELISA
偶联
unconjugated
克隆号
B6-A8
RRID
产品特性
形态
Liquid
浓度
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG1
纯化方式
Protein G affinity purified.
应用稀释度
-
ELISA
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1:10,000.
靶点
功能
Ebola virus VP40 Antibody is a high quality monoclonal Ebola virus VP40 antibody (also designated Ebola virus VP40 antibody) suitable for the detection of the Ebola virus VP40 protein of Ebola virus origin. Ebola virus VP40 Antibody (FE18) is available as the non-conjugated anti-Ebola virus VP40 antibody. Ebola virus is an enveloped, negative-stranded RNA virus which belongs to the family Filoviridae and has a distinctive morphology similar only to that of Marburg virus, but apparently has no antigenic characteristics of Marburg virus. Ebola virus is one of at least 18 known viruses capable of causing the viral hemorrhagic fever syndrome. Its genome is a nonsegmented RNA strand with seven structural proteins which include the major nucleoprotein (NP), glycoprotein (GP) and L protein, in addition to four other proteins: VP40, VPX, VP30 and VP24. All the proteins are encoded by monocistronic mRNA transcripts complementary to virion RNA. Only GP, the major protein of the surface spikes, is glycosylated.
背景文献
1. Pleet ML. et. al. Ebola VP40 in Exosomes Can Cause Immune Cell Dysfunction. Front Microbiol. 2016 Nov
UNIPROT
别名
Matrix protein VP40 antibody
Membrane-associated protein VP40 antibody
VP40 antibody
图片
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VP40 Antibody (HA601066) in indirect ELISA.
Indirect ELISA analysis of VP40 was performed by coating wells of a 96-well plate with 50 µl per well of VP40 antigen diluted in carbonate/bicarbonate buffer, at a concentration of 1 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with StartingBlock blocking buffer, and incubated with 50 µl per well of a mouse VP40 monoclonal antibody starting at a concentration of 20 µg/mL and serially diluting it to a concentration of 1.28 ng/mL for 2 hours at room temperature. The plate was washed and incubated with 50 µl per well of an HRP-conjugated goat anti-mouse IgG secondary antibody at a dilution of 1:15,000 for one hour at room temperature. Detection was performed using an Ultra TMB Substrate for 5 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
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