SARS-CoV-2 Nucleocapsid protein Mouse Monoclonal Antibody [A4G9] - BSA and Azide free (Detector)

The binding activity of HA600005 with Recombinant SARS-CoV-2 Nucleocapsid protein.
Immobilized Recombinant SARS-CoV-2 nucleocapsid protein at 1 μg/ml overnight at 4℃. Then blocked with 1% BSA for 1 hour at 37℃, and incubated with the primary antibody (HA600005) for 1 hour at 25℃. The EC50 of HA600005 is 47.22 ng/ml.

This antibody will detect SARS-CoV-2 Nucleocapsid protein in ELISA pair set (Clone: # A4G9-HRP). In a sandwich ELISA, it can be used as Detect antibody when paired with (Cat: # HA600007).

Sandwich ELISA analysis of SARS-CoV-2 Nucleocapsid protein matched pair antibodies

Capture: HA600008, SARS-CoV-2 Nucleocapsid protein Rabbit mAb [A5B8]
Detector: HA600005, SARS-CoV-2 Nucleocapsid protein Rabbit mAb [A4G9]

Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA600008) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant SARS-CoV-2 Nucleocapsid protein protein (HAg2002) starting from 4,000,000 pg/ml to 0 pg/ml and detect antibody (HA600005, Biotin, 0.5 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.