AP-4 complex subunit epsilon Rabbit Polyclonal Antibody
Catalog# HA500446
AP-4 complex subunit epsilon Rabbit Polyclonal Antibody
-
IF-Tissue
-
IHC-P
-
ELISA
-
Arabidopsis thaliana
-
unconjugated
概述
产品名称
AP-4 complex subunit epsilon Rabbit Polyclonal Antibody
抗体类型
Rabbit Polyclonal Antibody
免疫原
Synthetic peptide within ARATH AP-4 complex subunit epsilon aa 880-938 / 938.
种属反应性
Arabidopsis thaliana
验证应用
IF-Tissue, IHC-P, ELISA
分子量
104 kDa
阳性对照
A. thaliana tissue.
偶联
unconjugated
RRID
产品特性
形态
Liquid
浓度
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Immunogen affinity purified.
应用稀释度
-
IF-Tissue
-
1:50-1:100
-
IHC-P
-
1:50-1:200
-
ELISA
-
1:10,000
靶点
功能
Subunit of novel type of clathrin- or non-clathrin-associated protein coat involved in targeting proteins from the trans-Golgi network (TGN) to the endosomal-lysosomal system.
背景文献
1. Ivankovic D. et. al. Axonal autophagosome maturation defect through failure of ATG9A sorting underpins pathology in AP-4 deficiency syndrome. Autophagy. 2019 May 29:1-17.
2. De Pace R. et. al. Altered distribution of ATG9A and accumulation of axonal aggregates in neurons from a mouse model of AP-4 deficiency syndrome.PLoS Genet. 2018 Apr 26;14(4):e1007363.
亚细胞定位
Trans-Golgi network, coated pit.
UNIPROT
别名
AP-4 complex subunit epsilon
AP-4 adaptor complex subunit epsilon
Adaptor-related protein complex 4 subunit epsilon
Epsilon subunit of AP-4
Epsilon-adaptin
At1g31730
F27M3.7
图片
-
Immunofluorescence staining of paraffin- embedded A. thaliana using anti-AP-4 complex subunit epsilon rabbit polyclonal antibody.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with AP-4 complex subunit epsilon antibody at 1/50 dilution for 10 hours at 4℃ and detected using Alexa Fluor® 488 conjugate-Goat anti-Rabbit IgG (H+L) Secondary Antibody at a dilution of 1:500 for 1 hour at room temperature.
-
Immunohistochemical analysis of paraffin-embedded A. thaliana tissue using anti-AP-4 complex subunit epsilon antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500446, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
浙公网安备 33019202000643号