Cyclin D2 Rabbit Polyclonal Antibody
Catalog# HA500325
Cyclin D2 Rabbit Polyclonal Antibody
-
WB
-
Human
-
Mouse
-
Rat
概述
产品名称
Cyclin D2 Rabbit Polyclonal Antibody
抗体类型
Rabbit Polyclonal Antibody
免疫原
Synthetic peptide within human Cyclin D2 aa 30-79.
种属反应性
Human, Mouse, Rat
验证应用
WB
分子量
Predicted band size: 33 kDa
阳性对照
Mouse spleen tissue lysate, Hela cell lysate, rat spleen tissue lysates.
偶联
unconjugated
RRID
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Immunogen affinity purified.
应用稀释度
-
WB
-
1:500-1:1,000
靶点
功能
The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with CDK4 or CDK6 and functions as a regulatory subunit of the complex, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with and be involved in the phosphorylation of tumor suppressor protein Rb. Knockout studies of the homologous gene in mouse suggest the essential roles of this gene in ovarian granulosa and germ cell proliferation. High level expression of this gene was observed in ovarian and testicular tumors. Mutations in this gene are associated with megalencephaly-polymicrogyria-polydactyly-hydrocephalus syndrome 3 (MPPH3).
背景文献
1. Petkova SP. et. al. Cyclin D2-knock-out mice with attenuated dentate gyrus neurogenesis have robust deficits in long-term memory formation. Sci Rep. 2020 May
2. Luckey SW. et. al. Cyclin D2 is a critical mediator of exercise-induced cardiac hypertrophy. Exp Biol Med (Maywood). 2017 Dec
亚细胞定位
Nucleus, Cytoplasm, Membrane.
别名
CCND 2 antibody
ccnd2 antibody
CCND2_HUMAN antibody
CyclinD2 antibody
G1/S specific cyclin D2 antibody
G1/S-specific cyclin-D2 antibody
KIAK0002 antibody
MGC102758 antibody
MPPH3 antibody
图片
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Western blot analysis of Cyclin D2 on different lysates with Rabbit anti-Cyclin D2 antibody (HA500325) at 1/500 dilution.
Lane 1: Mouse spleen tissue lysate, 20 µg/Lane
Lane 2: Hela cell lysate, 10 µg/Lane
Predicted band size: 33 kDa
Observed band size: 30 kDa
Exposure time: 2 minutes;
8% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500325) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of Cyclin D2 on rat spleen tissue lysates with Rabbit anti-Cyclin D2 antibody (HA500325) at 1/500 dilution.
Lysates/proteins at 20 µg/Lane.
Predicted band size: 33 kDa
Observed band size: 30 kDa
Exposure time: 2 minutes;
8% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500325) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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