BTNL8 Rabbit Polyclonal Antibody
Catalog# HA500240
BTNL8 Rabbit Polyclonal Antibody
-
WB
-
IHC-P
-
Human
-
Mouse
-
Rat
-
unconjugated
概述
产品名称
BTNL8 Rabbit Polyclonal Antibody
抗体类型
Rabbit Polyclonal Antibody
免疫原
Synthetic peptide within human BTNL8 aa 480-500.
种属反应性
Human, Mouse, Rat
验证应用
WB, IHC-P
分子量
Predicted band size: 57 kDa
阳性对照
Rat brain tissue lysate, mouse testis tissue lysate, human stomach tissue.
偶联
unconjugated
RRID
产品特性
形态
Liquid
浓度
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Immunogen affinity purified.
应用稀释度
-
WB
-
1:500
-
IHC-P
-
1:100
靶点
功能
Human BTNL8 is a 500 amino acid (aa) type I transmembrane glycoprotein that contains a signal peptide followed by an extracellular domain (ECD), a transmembrane region and a short cytoplasmic domain. The ECD of human BTNL8 shares 88% sequence identity with the ECD of mouse BTNL8. BTNL8 has two alternatively spliced forms: B7-like and BTN-like. Both isoforms of BTNL8 are expressed in a range of human tissues. The complete immunological function of BTNL molecules is only beginning to emerge. BTNL8 has been shown to be important in initiation of primary immune responses, suggesting a role in priming of naïve T lymphocytes. Down-regulation of BNTL8 mRNA levels has been associated with ulcerative colitis and colon cancer. BTNL8 are expressed in colon, lung, testis and neutrophils, and its expression is significantly decreased in ulcerative colitis, colonic tumors as compared to unaffected tissue
背景文献
1. Chapoval AI. et. al. BTNL8, a butyrophilin-like molecule that costimulates the primary immune response. Mol Immunol. 2013 Dec
亚细胞定位
Membrane.
别名
Butyrophilin-like protein 8
BTNL8
UNQ702/PRO1347
图片
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Western blot analysis of BTNL8 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500240, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Rat brain tissue lysate
Lane 2: Mouse testis tissue lysate -
Immunohistochemical analysis of paraffin-embedded human stomach tissue using anti-BTNL8 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500240, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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