PRDC Rabbit Polyclonal Antibody
Catalog# HA500225
PRDC Rabbit Polyclonal Antibody
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WB
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FC
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Human
概述
产品名称
PRDC Rabbit Polyclonal Antibody
抗体类型
Rabbit Polyclonal Antibody
免疫原
Synthetic peptide within human PRDC aa 50-100.
种属反应性
Human
验证应用
WB, FC
分子量
Predicted band size: 19 kDa
阳性对照
CAL62 cell lysates, SH-SY5Y.
偶联
unconjugated
RRID
产品特性
形态
Liquid
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Immunogen affinity purified.
应用稀释度
-
WB
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1:500-1:1,000
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FC
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1:200-1:500
靶点
功能
PRDC (protein related to DAN and Cerberus) is a secreted cysteine knot-containing BMP antagonist belonging to the Cerberus/DAN (CAN) family. Mammalian CAN family members, including Gremlin, Dan, Cerberus, COCO, SOST, and USAG-1, have the conserved 6 cysteine residues that form a cysteine knot, and two additional cysteine residues located in the loops of the cysteine knot, which form an additional intrasubunit disulfide bond. PRDC acts as a specific BMP antagonist, binding to and blocking signaling induced by BMP-2 or -4, but not Activin or TGF-beta. Thus, PRDC expression in the ovary could be involved in follicular development by antagonizing the inhibitory effects of BMPs on FSH stimulation of progesterone
背景文献
1. Wen H. et. al. Grem2 mediates podocyte apoptosis in high glucose milieu. Biochimie. 2019 May
2. Ran A. et. al. GREM2 maintains stem cell-like phenotypes in gastric cancer cells by regulating the JNK signaling pathway. Cell Cycle. 2019 Oct
亚细胞定位
Secreted.
UNIPROT
别名
BMP antagonist 2 antibody
CKTSF1B2 antibody
Cysteine knot superfamily 1 antibody
Cysteine knot superfamily 1 BMP antagonist 2 antibody
DAN domain family member 3 antibody
DAND 3 antibody
DAND3 antibody
GREM 2 antibody
Grem2 antibody
GREM2_HUMAN antibody
展开图片
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Western blot analysis of PRDC on CAL62 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500225, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
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Flow cytometric analysis of PRDC was done on SH-SY5Y cells. The cells were fixed, permeabilized and stained with the primary antibody (HA500225, 1ug/ml) (red) compared with Rabbit IgG, monoclonal - Isotype Control (green). After incubation of the primary antibody at +4℃ for 1 hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃ (dark incubation).Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
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