CCL2 / MCP-1 Rabbit Polyclonal Antibody
Catalog# HA500042
CCL2 / MCP-1 Rabbit Polyclonal Antibody
-
WB
-
IHC-P
-
IF-Cell
-
Human
-
unconjugated
概述
产品名称
CCL2 / MCP-1 Rabbit Polyclonal Antibody
抗体类型
Rabbit Polyclonal Antibody
免疫原
Synthetic peptide within human CCL2 aa 50-99.
种属反应性
Human
验证应用
WB, IHC-P, IF-Cell
分子量
Predicted band size: 11 kDa
阳性对照
A549 cell lysate, Hela cell lysate, human colon tissue, human colon carcinoma tissue.
偶联
unconjugated
RRID
产品特性
形态
Liquid
浓度
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Immunogen affinity purified.
应用稀释度
-
WB
-
1:500
-
IHC-P
-
1:100-1:500
-
IF-Cell
-
1:50-1:200
靶点
功能
The chemokine (C-C motif) ligand 2 (CCL2) is also referred to as monocyte chemoattractant protein 1 (MCP1) and small inducible cytokine A2. CCL2 is a small cytokine that belongs to the CC chemokine family. CCL2 tightly regulates cellular mechanics and thereby recruits monocytes, memory T cells, and dendritic cells to the sites of inflammation produced by either tissue injury or infection. CCL2 is a monomeric polypeptide, with a molecular weight of approximately 13-15 kDa depending on levels of glycosylation. CCL2 is anchored in the plasma membrane of endothelial cells by glycosaminoglycan side chains of proteoglycans. CCL2 is primarily secreted by monocytes, macrophages and dendritic cells. Platelet derived growth factor is a major inducer of CCL2 gene.
背景文献
1. Li Y.S. et. al. The expression of monocyte chemotactic protein (MCP-1) in human vascular endothelium in vitro and in vivo. Mol. Cell. Biochem. 126:61-68(1993.
亚细胞定位
Secreted.
UNIPROT
别名
C-C motif chemokine 2 antibody
CCL2 antibody
CCL2_HUMAN antibody
Chemokine (C C motif) ligand 2 antibody
GDCF 2 antibody
GDCF-2 antibody
GDCF2 antibody
HC11 antibody
HSMCR30 antibody
JE antibody
展开图片
-
Western blot analysis of CCL2 / MCP-1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500042, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: A549 cell lysate
Lane 2: Hela cell lysate -
Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-CCL2 / MCP-1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500042, 1/800) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
-
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-CCL2 / MCP-1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500042, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
引文
-
Increased expression of the P2Y12 receptor is involved in the failure of autogenous arteriovenous fistula caused by stenosis
期刊: Renal Failure
DOI: 10.1080/0886022X.2023.2278314
IF: 3
应用: WB,IHC-P
反应种属: Human
发表时间: 2023 Nov
浙公网安备 33019202000643号