HA tag Recombinant Rabbit Multiclonal Antibody [PSH01-92] - BSA and Azide free
Catalog# HA750766
HA tag Recombinant Rabbit Multiclonal Antibody [PSH01-92] - BSA and Azide free
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WB
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IF-Cell
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IP
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Species independent
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HA721750
含抗保成分
-
unconjugated
概述
产品名称
HA tag Recombinant Rabbit Multiclonal Antibody [PSH01-92] - BSA and Azide free
抗体类型
Recombinant Rabbit multiclonal
免疫原
Synthetic peptide within N Terminal fusion protein and C Terminal fusion protein.
种属反应性
Species independent
验证应用
WB, IF-Cell, IP
阳性对照
N Terminal fusion protein and C Terminal fusion protein.
偶联
unconjugated
克隆号
PSH01-92
产品特性
形态
Liquid
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
PBS (pH7.4).
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
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WB
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1:5,000
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IF-Cell
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1:1,000
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IP
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1-2μg/sample
靶点
功能
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
背景文献
1. Liu Z et al. Mark4 promotes oxidative stress and inflammation via binding to PPAR and activating NF-kB pathway in mice adipocytes. Sci Rep 6:21382 (2016).
2. Gauson EJ et al. Evidence supporting a role for TopBP1 and Brd4 in the initiation but not continuation of human papillomavirus 16 E1/E2-mediated DNA replication. J Virol 89:4980-91 (2015).
别名
HA epitope tag antibody
HA1 antibody
HA2 antibody
hemagglutinin antibody
Hemagglutinin HA1 chain antibody
Hemagglutinin HA2 chain antibody
图片
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Western blot analysis of HA tag on different lysates with Rabbit anti-HA tag antibody (HA750766) at 1/5,000 dilution.
Lane 1: 293T cell lysate
Lane 2: 293T transfected with HA-tagged Nanos homolog 3 (N-terminal) cell lysate
Lane 3: 293T transfected with HA-tagged LIPT1 (N-terminal) cell lysate
Lane 4: 293T transfected with HA-tagged CXCL13 (C-terminal) cell lysate
Lane 5: 293T transfected with HA-tagged CLK4 (C-terminal) cell lysate
Lysates/proteins at 10 µg/Lane.
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750766) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunocytochemistry analysis of 293T cells labeling HA tag with Rabbit anti-HA tag antibody (HA750766) at 1/1,000 dilution.
293T cells, transfected with HA-tagged empty control, Nanos homolog 3 (N-terminal) or CLK4 (C-terminal) expression vector, respectively, were fixed in 4% paraformaldehyde for 10 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-HA tag antibody (HA750766) at 1/1,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
☑ Independent antibody validation (IAV)
HA tag was immunoprecipitated from 0.2 mg 293T transfected with HA-tagged Nanos homolog 3 (N-terminal) cell lysate with HA750766 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using M1008-1 at 1/20,000 dilution. Anti-Mouse IgG for IP Nano-secondary antibody (NBI02H) at 1/5,000 dilution was used for 1 hour at room temperature.
Lane 1: 293T transfected with HA-tagged Nanos homolog 3 (N-terminal) cell lysate (input)
Lane 2: HA750766 IP in 293T transfected with HA-tagged Nanos homolog 3 (N-terminal) cell lysate
Lane 3: Mouse IgG instead of HA750766 in 293T transfected with HA-tagged Nanos homolog 3 (N-terminal) cell lysate
Blocking/Dilution buffer: 5% NFDM/TBST
Exposure time: 35 seconds; ECL: K1801
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"