Granzyme B Recombinant Rabbit Monoclonal Antibody [PSH14-20]
Rmb: 699 特惠 1500
Catalog# HA723609
Granzyme B Recombinant Rabbit Monoclonal Antibody [PSH14-20]
-
WB
-
Mouse
-
HA751513
不含抗保成分
-
unconjugated
概述
产品名称
Granzyme B Recombinant Rabbit Monoclonal Antibody [PSH14-20]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein within Mouse Granzyme B aa 21-247.
种属反应性
Mouse
验证应用
WB
靶点分子量
Predicted band size: 27 kDa
阳性对照
CTLL-2 cell lysates.
偶联
unconjugated
克隆号
PSH14-20
反应性数据
| WB | |
|---|---|
| Mouse |
|
| Human |
|
| Rat |
|
产品特性
形态
Liquid
浓度
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
存储缓冲液
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
-
1:100,000
靶点
功能
Abundant protease in the cytosolic granules of cytotoxic T-cells and NK-cells which activates caspase-independent pyroptosis when delivered into the target cell through the immunological synapse . It cleaves after Asp. Once delivered into the target cell, acts by catalyzing cleavage of gasdermin-E (GSDME), releasing the pore-forming moiety of GSDME, thereby triggering pyroptosis and target cell death . Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis .
背景文献
1. Gleave A et al. Granzyme B in Autoimmune Skin Disease. Biomolecules. 2023 Feb
2. Jung K et al. Granzyme B in epithelial barrier dysfunction and related skin diseases. Am J Physiol Cell Physiol. 2022 Jul
亚细胞定位
Secreted, Cytolytic granule.
UNIPROT
别名
C11 antibody
Cathepsin G like 1 antibody
Cathepsin G-like 1 antibody
CCPI antibody
CGL 1 antibody
CGL1 antibody
CSP B antibody
CSPB antibody
CTLA 1 antibody
CTLA-1 antibody
展开图片
-
Western blot analysis of Granzyme B on CTLL-2 cell lysates with Rabbit anti-Granzyme B antibody (HA723609) at 1/100,000 dilution.
Lysates/proteins at 20 µg/Lane.
Predicted band size: 27 kDa
Observed band size: 35 kDa
Exposure time: 4 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723609) at 1/100,000 dilution was used in primary antibody dilution (K1803) at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
浙公网安备 33019202000643号