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GST Mouse Monoclonal Antibody [16-21]

RMB: 1500

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Catalog# EM80701

GST Mouse Monoclonal Antibody [16-21]

Application

  • WB

  • ELISA

Reactivity

  • Species independent

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

概述

产品名称

GST Mouse Monoclonal Antibody [16-21]

抗体类型

Mouse Monoclonal Antibody

免疫原

Recombinant protein within GST protein.

种属反应性

Species independent

验证应用

WB, ELISA

阳性对照

GST-tagged recombinant protein.

偶联

unconjugated

克隆号

16-21

RRID

AB_2940896

产品特性

形态

Liquid

存放说明

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

存储缓冲液

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

亚型

IgG1

纯化方式

Protein A affinity purified.

应用稀释度

  • WB

  • 1:5,000

  • ELISA

  • 1:10,000-1:20,000

靶点

功能

The glutathione S-transferase (GST) family of enzymes comprises a long list of cytosolic, mitochondrial, and microsomal proteins that are capable of multiple reactions with a multitude of substrates, both endogenous and xenobiotic. Mammalian cytosolic GSTs are dimeric both subunits being from the same class of GSTs. The monomers are in the range of 22–29 kDa. Glutathione S-transferase is used to create the “GST gene fusion system” in genetic engineering. Here, GST is used to purify and detect proteins of interest.

背景文献

1. Choi, H.K., et al. 2013. Early estrogen-induced gene 1, a novel RANK signaling component, is essential for osteoclastogenesis. Cell Res. 23: 524-536.

2. Patra, A.K., et al. 2013. An alternative NFAT-activation pathway mediated by IL-7 is critical for early thymocyte development. Nat. Immunol. 14: 127-135.

别名

Glutathione S Transferase antibody

Glutathione S-transferase class-mu 26 kDa isozyme antibody

GST antibody

Sj26 antigen antibody

SjGST antibody

图片

  • Western blot analysis of GST on different lysates with Mouse anti-GST antibody (<a href="/products/EM80701" style="font-weight: bold;text-decoration: underline;">EM80701</a>) at 1/5,000 dilution.<br /><br />Lane 1: 293T cell lysate<br />Lane 2: 293T transfected with GST-tagged Histone H3.1 cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Exposure time: 46 seconds;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/EM80701" style="font-weight: bold;text-decoration: underline;">EM80701</a>) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of GST on different lysates with Mouse anti-GST antibody (EM80701) at 1/5,000 dilution.

    Lane 1: 293T cell lysate
    Lane 2: 293T transfected with GST-tagged Histone H3.1 cell lysate

    Lysates/proteins at 10 µg/Lane.

    Exposure time: 46 seconds;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM80701) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

引文

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