GITR Recombinant Rabbit Monoclonal Antibody [PSH04-06]
Catalog# HA722087
GITR Recombinant Rabbit Monoclonal Antibody [PSH04-06]
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WB
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IF-Cell
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FC
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Human
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HA750929
不含抗保成分
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unconjugated
概述
产品名称
GITR Recombinant Rabbit Monoclonal Antibody [PSH04-06]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein within human GITR aa 1-162 / 241.
种属反应性
Human
验证应用
WB, IF-Cell, FC
分子量
Predicted band size: 26 kDa
阳性对照
HUT 102 cell lysates, HUT 102.
偶联
unconjugated
克隆号
PSH04-06
RRID
反应性数据
| WB | IF-Cell | FC | |
|---|---|---|---|
| Human |
|
|
|
| Mouse |
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| Rat |
|
产品特性
形态
Liquid
浓度
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
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WB
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1:1,000
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IF-Cell
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1:200
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FC
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1:1,000
靶点
功能
Tumor necrosis factor receptor superfamily member 18 (TNFRSF18), also known as glucocorticoid-induced TNFR-related protein (GITR) or CD357. GITR is encoded and tnfrsf18 gene at chromosome 4 in mice. GITR is type I transmembrane protein and is described in 4 different isoforms. GITR human orthologue, also called activation-inducible TNFR family receptor (AITR), is encoded by the TNFRSF18 gene at chromosome 1. GITR is a member of TNFR superfamily and shares high homology in cytoplasmic domain, characterized with cysteine pseudo-repeats, with other members of TNFRSF, such as CD137, OX40 or CD27. GITR is constitutively expressed on CD25+CD4+ regulatory T cells and its expression is upregulated on all T cell subsets after activation. GITR is also expressed on murine neutrophils and NK cells. GITR interacts with its ligand (GITRL) that is expressed on antigen-presenting cells (APC) and endothelial cells.
背景文献
1. Ke S et al. High-level of intratumoral GITR+ CD4 T cells associate with poor prognosis in gastric cancer. iScience. 2022 Nov
2. Tian J et al. The Role of GITR/GITRL Interaction in Autoimmune Diseases. Front Immunol. 2020 Oct
亚细胞定位
Cell membrane; Secreted.
UNIPROT
别名
Activation inducible TNFR family receptor antibody
Activation-inducible TNFR family receptor antibody
AITR antibody
CD357 antibody
GITR antibody
GITR D antibody
GITR-D antibody
GITRD antibody
Glucocorticoid induced TNFR related protein antibody
glucocorticoid-induced tnf receptor ligand antibody
展开图片
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Western blot analysis of GITR on HUT 102 cell lysates with Rabbit anti-GITR antibody (HA722087) at 1/1,000 dilution.
Lysates/proteins at 20 µg/Lane.
Predicted band size: 26 kDa
Observed band size: 26 kDa
Exposure time: 46 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722087) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunocytochemistry analysis of HUT 102 cells labeling GITR with Rabbit anti-GITR antibody (HA722087) at 1/200 dilution.
Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-GITR antibody (HA722087) at 1/200 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
Flow cytometric analysis of HUT 102 cells labeling GITR.
Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA722087, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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