Fibronectin Recombinant Rabbit Monoclonal Antibody [JF0582] - BSA and Azide free
Rmb: 5600
Catalog# HA750340
Fibronectin Recombinant Rabbit Monoclonal Antibody [JF0582] - BSA and Azide free
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WB
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IF-Cell
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IF-Tissue
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IHC-P
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Human
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Mouse
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ET1702-25
含抗保成分
-
unconjugated
概述
产品名称
Fibronectin Recombinant Rabbit Monoclonal Antibody [JF0582] - BSA and Azide free
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant full length protein of Human Fibronectin aa 1-2477 / 2477.
种属反应性
Human, Mouse
验证应用
WB, IF-Cell, IF-Tissue, IHC-P
靶点分子量
Predicted band size: 272 kDa
阳性对照
HepG2 cell lysate, Caco-2 cell lysate, SK-MEL-28 cell lysate, human kidney tissue lysate, MEF cell lysate, NIH/3T3 cell lysate, MG-63 cell lysates, HepG2, Hela, NIH/3T3, mouse liver tissue.
偶联
unconjugated
克隆号
JF0582
产品特性
形态
Liquid
浓度
存放说明
Store at 2-8℃. Avoid freeze.
存储缓冲液
1*PBS (pH7.4).
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
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WB
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1:1,000-1:2,000
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IF-Cell
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1:100-1:500
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IF-Tissue
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1:100-1:500
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IHC-P
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1:50-1:200
靶点
功能
Fibronectin is an extracellular matrix glycoprotein present on most cell surfaces, in extracellular fluids and in plasma. A high molecular weight heterodimeric protein, it was originally discovered as a protein missing from the surfaces of virus-transformed cells, and it has been shown to be involved in various functions including cell adhesion, cell motility and wound healing. Alternative splicing and glycosylation give rise to several different forms of Fibronectin, some of which exhibit restricted tissue distribution or association with malignancies. It has been shown that Myofibroblast phenotype formation correlates with the occurrence of glycosylated Fibronectin and Fibronectin splice variants in Dupuytren's disease.
背景文献
1. Patten J et al. Fibronectin in development and wound healing. Adv Drug Deliv Rev. 2021 Mar
2. Dalton CJ et al. Fibronectin: Molecular Structure, Fibrillar Structure and Mechanochemical Signaling. Cells. 2021 Sep
组织特异性
Expressed in the inner limiting membrane and around blood vessels in the retina (at protein level). Plasma FN (soluble dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. Ugl-Y1, Ugl-Y2 and Ugl-Y3 are found in urine.
翻译后修饰
Sulfated.; It is not known whether both or only one of Thr-2155 and Thr-2156 are/is glycosylated.; Forms covalent cross-links mediated by a transglutaminase, such as F13A or TGM2, between a glutamine and the epsilon-amino group of a lysine residue, forming homopolymers and heteropolymers (e.g. fibrinogen-fibronectin, collagen-fibronectin heteropolymers).; Phosphorylated by FAM20C in the extracellular medium.; Proteolytic processing produces the C-terminal NC1 peptide, anastellin.; Some lysine residues are oxidized to allysine by LOXL3, promoting fibronectin activation and matrix formation.
亚细胞定位
Secreted, extracellular space, extracellular matrix.
别名
CIG antibody
Cold insoluble globulin antibody
Cold-insoluble globulin antibody
DKFZp686F10164 antibody
DKFZp686H0342 antibody
DKFZp686I1370 antibody
DKFZp686O13149 antibody
ED B antibody
Fibronectin 1 antibody
FINC antibody
展开图片
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Western blot analysis of Fibronectin on different lysates with Rabbit anti-Fibronectin antibody (HA750340) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution.
Lane 1: HepG2 cell lysate
Lane 2: Caco-2 cell lysate
Lane 3: SK-MEL-28 cell lysate
Lane 4: Human kidney tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 272 kDa
Observed band size: 272 kDa
Exposure time: 3 minutes 20 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750340) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of Fibronectin on different lysates with Rabbit anti-Fibronectin antibody (HA750340) at 1/2,000 dilution.
Lane 1: HepG2 cell lysate
Lane 2: MEF cell lysate
Lane 3: NIH/3T3 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 272 kDa
Observed band size: 272 kDa
Exposure time: 2 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750340) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of Fibronectin on MG-63 cell lysates with Rabbit anti-Fibronectin antibody (HA750340) at 1/1,000 dilution.
Lysates/proteins at 10 µg/Lane.
Predicted band size: 272 kDa
Observed band size: 272 kDa
Exposure time: 2 minutes;
6% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750340) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/100,000 dilution was used for 1 hour at room temperature. -
ICC staining of Fibronectin in HepG2 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (HA750340, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
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ICC staining of Fibronectin in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (HA750340, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
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ICC staining of Fibronectin in NIH/3T3 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (HA750340, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
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Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-Fibronectin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750340, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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☑ Knockdown (KD)
Western blot analysis of Fibronectin on different lysates with Rabbit anti-Fibronectin antibody (HA750340) at 1/2,000 dilution.
Lane 1: HepG2-si NT cell lysate
Lane 2: HepG2-si Fibronectin cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 272 kDa
Observed band size: 272 kDa
Exposure time: 20 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750340) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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