FABP4 Recombinant Rabbit Monoclonal Antibody [JM10-99] - BSA and Azide free
Catalog# HA750398
FABP4 Recombinant Rabbit Monoclonal Antibody [JM10-99] - BSA and Azide free
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WB
-
IF-Cell
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IHC-Fr
-
Human
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Mouse
-
Rat
概述
产品名称
FABP4 Recombinant Rabbit Monoclonal Antibody [JM10-99] - BSA and Azide free
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Synthetic peptide within human FABP4 aa 60-100.
种属反应性
Human, Mouse, Rat
验证应用
WB, IF-Cell, IHC-Fr
分子量
Predicted band size: 15 kDa
阳性对照
Mouse white adipose tissue lysate, Mouse brown adipose tissue lysate, Mouse kidney tissue lysate, Rat white adipose tissue lysate, Rat brown adipose tissue lysate, Rat kidney tissue lysate, HAEC.
偶联
unconjugated
克隆号
JM10-99
产品特性
形态
Liquid
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
PBS (pH7.4).
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
-
1:5,000
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IF-Cell
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1:100-1:500
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IHC-Fr
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1
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100
靶点
功能
Fatty acid-binding proteins, designated FABPs, are a family of homologous, cytoplasmic proteins that are expressed in a highly tissue-specific manner and play an integral role in the balance between lipid and carbohydrate metabolism. FABPs mediate fatty acid (FA) and/or hydrophobic ligand uptake, transport, and targeting within their respective tissues. The mechanisms underlying these actions can give rise to both passive diffusional uptake and protein-mediated transmembrane transport of FAs. FABPs are expressed in adipocytes (A-FABP), brain (B-FABP), epidermis (E-FABP, also designated psoriasis-associated FABP or PA-FABP), muscle and heart (H-FABP, also designated mammary-derived growth inhibitor or MDGI), intestine (I-FABP), liver (L-FABP), myelin (M-FABP) and testis (T-FABP). The human A-FABP gene is organized into 4 exons, maps to chromosome 8q21, and encodes a 132-amino acid protein. A-FABP protein comprises approximately 1% of the total cytosolic protein in human adipose tissue.
背景文献
1. Roberts JN et al. Dynamic Surfaces for the Study of Mesenchymal Stem Cell Growth through Adhesion Regulation. ACS Nano 10:6667-79 (2016).
2. Ohyama Y et al. Differential expression of fatty acid-binding proteins and pathological implications in the progression of tongue carcinoma. Mol Clin Oncol 2:19-25 (2014).
序列相似性
Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family.
亚细胞定位
Nucleus. Cytoplasm.
别名
3T3-L1 lipid-binding protein antibody
422/aP2 antibody
A-FABP antibody
adipocyte antibody
Adipocyte lipid binding protein antibody
Adipocyte lipid-binding protein antibody
Adipocyte protein AP2 antibody
Adipocyte-type fatty acid-binding protein antibody
AFABP antibody
ALBP antibody
展开图片
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☑ Relative expression (RE)
Western blot analysis of FABP4 on different lysates with Rabbit anti-FABP4 antibody (HA750398) at 1/5,000 dilution and competitor's antibody at 1/5,000 dilution.
Lane 1: Mouse white adipose tissue lysate
Lane 2: Mouse brown adipose tissue lysate
Lane 3: Mouse kidney tissue lysate (negative)
Lane 4: Rat white adipose tissue lysate
Lane 5: Rat brown adipose tissue lysate
Lane 6: Rat kidney tissue lysate (negative)
Lysates/proteins at 10 µg/Lane.
Predicted band size: 15 kDa
Observed band size: 15 kDa
Exposure time: 3 minutes 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750398) at 1/5,000 dilution and competitor's antibody at 1/5,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunocytochemistry analysis of HAEC cells labeling FABP4 with Rabbit anti-FABP4 antibody (HA750398) at 1/500 dilution.
Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-FABP4 antibody (HA750398) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.n. -
Immunofluorescence analysis of frozen mouse adipose tissue labeling FABP4 with Rabbit anti-FABP4 antibody (HA750398).
The tissues were blocked in 3% BSA for 30 minutes at room temperature, washed with PBS, and then probed with the primary antibody (HA750398, green) at 1/100 dilution overnight at 4℃, washed with PBS. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/200 dilution. Nuclei were counterstained with DAPI (blue). Image acquisition was performed with KFBIO KF-FL-400 Scanner.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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