ErbB3/HER3 Recombinant Rabbit Monoclonal Antibody [PD00-44]
Catalog# HA721194
ErbB3/HER3 Recombinant Rabbit Monoclonal Antibody [PD00-44]
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IHC-P
-
IF-Tissue
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Human
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unconjugated
概述
产品名称
ErbB3/HER3 Recombinant Rabbit Monoclonal Antibody [PD00-44]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Synthetic peptide within Human ErbB3 aa 650-750 (internal sequence).
种属反应性
Human
验证应用
IHC-P, IF-Tissue
分子量
Predicted band size: 148 kDa
阳性对照
Human small intestine tissue.
偶联
unconjugated
克隆号
PD00-44
RRID
产品特性
形态
Liquid
浓度
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
IHC-P
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1:1,000-1:2,000
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IF-Tissue
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1:200
靶点
功能
This gene encodes a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. This membrane-bound protein has a neuregulin binding domain but not an active kinase domain. It therefore can bind this ligand but not convey the signal into the cell through protein phosphorylation. However, it does form heterodimers with other EGF receptor family members which do have kinase activity. Heterodimerization leads to the activation of pathways which lead to cell proliferation or differentiation. Amplification of this gene and/or overexpression of its protein have been reported in numerous cancers, including prostate, bladder, and breast tumors. Alternate transcriptional splice variants encoding different isoforms have been characterized. One isoform lacks the intermembrane region and is secreted outside the cell. This form acts to modulate the activity of the membrane-bound form. Additional splice variants have also been reported, but they have not been thoroughly characterized.
背景文献
1. Eliseev IE et al. Targeting ErbB3 Receptor in Cancer with Inhibitory Antibodies from Llama. Biomedicines. 2021 Aug
2. Kiavue N et al. ERBB3 mutations in cancer: biological aspects, prevalence and therapeutics. Oncogene. 2020 Jan
亚细胞定位
Cell membrane, Secreted.
UNIPROT
别名
c erbB 3 antibody
c erbB3 antibody
Erb b2 receptor tyrosine kinase 3 antibody
ErbB 3 antibody
ERBB3 antibody
ERBB3 protein antibody
erbB3 S antibody
ERBB3_HUMAN antibody
Glial growth factor receptor antibody
HER 3 antibody
展开图片
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Immunohistochemical analysis of paraffin-embedded human small intestine tissue with Rabbit anti-ErbB3/HER3 antibody (HA721194) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721194) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-ErbB3/HER3 antibody (HA721194) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721194) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunofluorescence analysis of paraffin-embedded human small intestine tissue labeling ErbB3/HER3 with Rabbit anti-ErbB3/HER3 antibody (HA721194) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA721194, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
引文
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Identification of a Hypoxia-Related Signature for Predicting Prognosis and the Immune Microenvironment in Bladder Cancer
期刊: Frontiers In Molecular Biosciences
DOI:
IF: 5.25
应用: IHC-P
反应种属: Human
发表时间: 2021 May
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