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ACSL4 / FACL4 Recombinant Rabbit Monoclonal Antibody [JE56-33]

RMB: 900 特惠 1500

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Catalog# ET7111-43

ACSL4 / FACL4 Recombinant Rabbit Monoclonal Antibody [JE56-33]

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

BSA and Azide free
Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

概述

产品名称

ACSL4 / FACL4 Recombinant Rabbit Monoclonal Antibody [JE56-33]

抗体类型

Recombinant Rabbit monoclonal Antibody

免疫原

Recombinant protein within human FACL4 aa 561-711 (Cytoplasmic).

种属反应性

Human, Mouse, Rat

验证应用

WB, IHC-P

分子量

Predicted band size: 79 kDa

阳性对照

PC-12 cell lysate, C6 cell lysate, L6 cell lysate, Rat brain tissue lysate, Rat lung tissue lysate, Rat thymus tissue lysate, A549 cell lysate, Mouse brain tissue lysate, mouse brain tissue, mouse lung tissue, rat brain tissue.

偶联

unconjugated

克隆号

JE56-33

RRID

AB_3071100

产品特性

形态

Liquid

浓度

存放说明

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

存储缓冲液

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

亚型

IgG

纯化方式

Protein A affinity purified.

应用稀释度

  • WB

  • 1:1,000-1:5,000

  • IHC-P

  • 1:1,000

靶点

功能

The protein encoded by this gene is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. This isozyme preferentially utilizes arachidonate as substrate. The absence of this enzyme may contribute to the cognitive disability or Alport syndrome. Alternative splicing of this gene generates multiple transcript variants.

背景文献

1. Cheng J. et. al. ACSL4 suppresses glioma cells proliferation via activating ferroptosis. Oncol Rep. 2020 Jan

2. Kuwata H. et. al. Role of acyl-CoA synthetase ACSL4 in arachidonic acid metabolism. Prostaglandins Other Lipid Mediat. 2019 Oct

亚细胞定位

Peroxisome membrane, Mitochondrion outer membrane, Microsome membrane, Endoplasmic reticulum membrane, Cell membrane.

UNIPROT

SwissProt: O60488 Human

SwissProt: Q9QUJ7 Mouse

SwissProt: O35547 Rat

别名

ACS 4 antibody

ACS4 antibody

ACSL 4 antibody

Acsl4 antibody

ACSL4_HUMAN antibody

acyl CoA synthetase 4 antibody

Acyl CoA synthetase long chain family member 4 antibody

FACL 4 antibody

FACL4 antibody

Fatty acid Coenzyme A ligase antibody

展开

图片

  • <span style="font-weight: bold;">☑ Knockdown (KD)</span><br /><br />Western blot analysis of ACSL4 / FACL4 on different lysates with Rabbit anti-ACSL4 / FACL4 antibody (<a href="/products/ET7111-43" style="font-weight: bold;text-decoration: underline;">ET7111-43</a>) at 1/5,000 dilution.<br /><br />Lane 1: A549-si NT cell lysate<br />Lane 2: A549-si ACSL4 / FACL4 cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 79 kDa<br />Observed band size: 75 kDa<br /><br />Exposure time: 1 minute; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET7111-43" style="font-weight: bold;text-decoration: underline;">ET7111-43</a>) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Knockdown (KD)

    Western blot analysis of ACSL4 / FACL4 on different lysates with Rabbit anti-ACSL4 / FACL4 antibody (ET7111-43) at 1/5,000 dilution.

    Lane 1: A549-si NT cell lysate
    Lane 2: A549-si ACSL4 / FACL4 cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 79 kDa
    Observed band size: 75 kDa

    Exposure time: 1 minute; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7111-43) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of ACSL4 / FACL4 on different lysates with Rabbit anti-ACSL4 / FACL4 antibody (<a href="/products/ET7111-43" style="font-weight: bold;text-decoration: underline;">ET7111-43</a>) at 1/1,000 dilution.<br /><br />Lane 1: PC-12 cell lysate<br />Lane 2: C6 cell lysate<br />Lane 3: L6 cell lysate<br />Lane 4: Rat brain tissue lysate<br />Lane 5: Rat lung tissue lysate<br />Lane 6: Rat thymus tissue lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 79 kDa<br />Observed band size: 75 kDa<br /><br />Exposure time: 42 seconds; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET7111-43" style="font-weight: bold;text-decoration: underline;">ET7111-43</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of ACSL4 / FACL4 on different lysates with Rabbit anti-ACSL4 / FACL4 antibody (ET7111-43) at 1/1,000 dilution.

    Lane 1: PC-12 cell lysate
    Lane 2: C6 cell lysate
    Lane 3: L6 cell lysate
    Lane 4: Rat brain tissue lysate
    Lane 5: Rat lung tissue lysate
    Lane 6: Rat thymus tissue lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 79 kDa
    Observed band size: 75 kDa

    Exposure time: 42 seconds; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7111-43) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of ACSL4 / FACL4 on different lysates with Rabbit anti-ACSL4 / FACL4 antibody (<a href="/products/ET7111-43" style="font-weight: bold;text-decoration: underline;">ET7111-43</a>) at 1/1,000 dilution.<br /><br />Lane 1: A549 cell lysate (20 µg/Lane)<br />Lane 2: Mouse brain tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 79 kDa<br />Observed band size: 75 kDa<br /><br />Exposure time: 25 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET7111-43" style="font-weight: bold;text-decoration: underline;">ET7111-43</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of ACSL4 / FACL4 on different lysates with Rabbit anti-ACSL4 / FACL4 antibody (ET7111-43) at 1/1,000 dilution.

    Lane 1: A549 cell lysate (20 µg/Lane)
    Lane 2: Mouse brain tissue lysate (40 µg/Lane)

    Predicted band size: 79 kDa
    Observed band size: 75 kDa

    Exposure time: 25 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7111-43) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-ACSL4 / FACL4 antibody (<a href="/products/ET7111-43" style="font-weight: bold;text-decoration: underline;">ET7111-43</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET7111-43" style="font-weight: bold;text-decoration: underline;">ET7111-43</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-ACSL4 / FACL4 antibody (ET7111-43) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7111-43) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse lung tissue with Rabbit anti-ACSL4 / FACL4 antibody (<a href="/products/ET7111-43" style="font-weight: bold;text-decoration: underline;">ET7111-43</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET7111-43" style="font-weight: bold;text-decoration: underline;">ET7111-43</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue with Rabbit anti-ACSL4 / FACL4 antibody (ET7111-43) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7111-43) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-ACSL4 / FACL4 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET7111-43" style="font-weight: bold;text-decoration: underline;">ET7111-43</a>, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-ACSL4 / FACL4 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7111-43, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

引文

同靶点 & 同通路的产品

metafield image

ACSL4 / FACL4 Recombinant Rabbit Monoclonal Antibody [JE56-33] - BSA and Azide free

Application: WB,IHC-P

Reactivity: Human,Mouse,Rat

Conjugate: unconjugated

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