APLP2 Recombinant Rabbit Monoclonal Antibody [JE54-62]
Catalog# ET7110-78
APLP2 Recombinant Rabbit Monoclonal Antibody [JE54-62]
-
WB
-
IHC-P
-
Human
-
Rat
-
unconjugated
概述
产品名称
APLP2 Recombinant Rabbit Monoclonal Antibody [JE54-62]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein within Human APLP2 aa 201-300 / 763.
种属反应性
Human, Rat
验证应用
WB, IHC-P
分子量
Predicted band size: 87 kDa
阳性对照
Rat brian tissue lysate, human placenta tissue lysate, Hela cell lysate, rat testis tissue, human kidney tissue.
偶联
unconjugated
克隆号
JE54-62
RRID
产品特性
形态
Liquid
浓度
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
-
1:500-1:1,000
-
IHC-P
-
1:200-1:1,000
靶点
功能
This gene encodes amyloid precursor- like protein 2 (APLP2), which is a member of the APP (amyloid precursor protein) family including APP, APLP1 and APLP2. This protein is ubiquitously expressed. It contains heparin-, copper- and zinc- binding domains at the N-terminus, BPTI/Kunitz inhibitor and E2 domains in the middle region, and transmembrane and intracellular domains at the C-terminus. This protein interacts with major histocompatibility complex (MHC) class I molecules. The synergy of this protein and the APP is required to mediate neuromuscular transmission, spatial learning and synaptic plasticity. This protein has been implicated in the pathogenesis of Alzheimer's disease. Multiple alternatively spliced transcript variants encoding different isoforms have been identified.
背景文献
1. Gao L. et. al. Role of APLP2 in the prognosis and clinicopathology of renal cell carcinoma. Oncol Lett. 2019 Jan.
2. Wang X. et. al. APLP2 Modulates JNK-Dependent Cell Migration in Drosophila. Biomed Res Int. 2018 Jul.
序列相似性
Belongs to the APP family.
组织特异性
Expressed in placenta, brain, heart, lung, liver, kidney and endothelial tissues.
翻译后修饰
The BPTI/Kunitz inhibitor domain is O-glycosylated.
亚细胞定位
Nucleus, cell membrane.
别名
amyloid beta (A4) precursor like protein 2 antibody
Amyloid like protein 2 antibody
Amyloid precursor protein homolog HSD 2 antibody
amyloid precursor protein homolog HSD2 antibody
Amyloid protein homolog antibody
Amyloid-like protein 2 antibody
APLP-2 antibody
Aplp2 antibody
APLP2_HUMAN antibody
APPH antibody
展开图片
-
☑ Knockdown (KD)
Western blot analysis of APLP2 on different lysates with Rabbit anti-APLP2 antibody (ET7110-78) at 1/1,000 dilution.
Lane 1: A549-WT cell lysate
Lane 2: A549-KD APLP2 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 87 kDa
Observed band size: 100 kDa
Exposure time: 2 minutes 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7110-78) at 1/1,000 dilution was used in 5% BSA at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of APLP2 on different lysates with Rabbit anti-APLP2 antibody (ET7110-78) at 1/500 dilution.
Lane 1: Rat brian tissue lysate(20 µg/Lane)
Lane 2: Human placenta tissue lysate(20 µg/Lane)
Lane 3: Hela cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 87 kDa
Observed band size: 100/120 kDa
Exposure time: 2 minutes;
8% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7110-78) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded rat testis tissue with Rabbit anti-APLP2 antibody (ET7110-78) at 1/50 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-78) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-APLP2 antibody (ET7110-78) at 1/50 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-78) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
浙公网安备 33019202000643号