NEK6 Recombinant Rabbit Monoclonal Antibody [JE51-72]
Rmb: 980 特惠 1500
Catalog# ET7110-15
NEK6 Recombinant Rabbit Monoclonal Antibody [JE51-72]
-
WB
-
Human
-
unconjugated
概述
产品名称
NEK6 Recombinant Rabbit Monoclonal Antibody [JE51-72]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein within Human NEK6 aa 170-313 / 313.
种属反应性
Human
验证应用
WB
靶点分子量
Predicted band size: 36 kDa
阳性对照
THP-1 cell lysate, Wild-type 293T whole cell lysate.
偶联
unconjugated
克隆号
JE51-72
RRID
产品特性
形态
Liquid
浓度
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
存储缓冲液
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
-
1:500-1:2,000
靶点
功能
Serine/threonine-protein kinase Nek6 is an enzyme that in humans is encoded by the NEK6 gene. The Aspergillus nidulans 'never in mitosis A' (NIMA) gene encodes a serine/threonine kinase that controls initiation of mitosis. NIMA-related kinases (NEKs) are a group of protein kinases that are homologous to NIMA. Evidence suggests that NEKs perform functions similar to those of NIMA. It is a protein kinase which plays an important role in mitotic cell cycle progression. Required for chromosome segregation at metaphase-anaphase transition, robust mitotic spindle formation and cytokinesis. Phosphorylates ATF4, CIR1, PTN, RAD26L, RBBP6, RPS7, RPS6KB1, TRIP4, STAT3 and histones H1 and H3. Phosphorylates KIF11 to promote mitotic spindle formation. Involved in G2/M phase cell cycle arrest induced by DNA damage. Inhibition of activity results in apoptosis. May contribute to tumorigenesis by suppressing p53/TP53-induced cancer cell senescence.
背景文献
1. Jee H.J. et. al. Nek6 overexpression antagonizes p53-induced senescence in human cancer cells. Cell Cycle 9:4703-4710(2010).
2. Vaz Meirelles G. et. al. Characterization of hNek6 interactome reveals an important role for its short N-terminal domain and colocalization with proteins at the centrosome. J. Proteome Res. 9:6298-6316(2010).
序列相似性
Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.
组织特异性
Ubiquitous, with highest expression in heart and skeletal muscle.
翻译后修饰
Autophosphorylated. Phosphorylation at Ser-206 is required for its activation. Phosphorylated upon IR or UV-induced DNA damage. Phosphorylated by CHEK1 and CHEK2. Interaction with APBB1 down-regulates phosphorylation at Thr-210.
亚细胞定位
Cytoplasm, Cytoskeleton, Microtubule, Nucleus.
UNIPROT
别名
Highly similar to cell cycle protein kinase CDC5/MSD2 antibody
NEK6 antibody
NEK6_HUMAN antibody
Never in mitosis A-related kinase 6 antibody
NIMA (Never In Mitosis Gene A) Related Kinase 6 antibody
NimA related protein kinase 6 antibody
NimA-related protein kinase 6 antibody
Protein kinase SID6 1512 antibody
Protein kinase SID6-1512 antibody
Putative serine threonine protein kinase antibody
展开图片
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Western blot analysis of NEK6 on different lysates with Rabbit anti-NEK6 antibody (ET7110-15) at 1/2,000 dilution.
Lane 1: HeLa (Human cervical adenocarcinoma cell) (15 µg/Lane)
Lane 2: THP-1 (Human acute monoblastic leukemia cell) (15 µg/Lane)
Lane 3: Hep G2 (Human hepatocellular carcinoma cell) (15 µg/Lane)
Lane 4: HEK-293 (Human embryonic kidney cell) (15 µg/Lane)
Lane 5: Mouse brain tissue lysate (30 µg/Lane)
Lane 6: Rat brain tissue lysate (30 µg/Lane)
Exposure time: 180 seconds; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: ET7110-15, 1/2,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 36 kDa
Observed band size: 36 kDa -
☑ Knockout (KO)
All lanes: Western blot analysis of NEK6 with anti-NEK6 antibody[JE51-72] (ET7110-15) at 1/1,000 dilution.
Lane 1: Wild-type 293T whole cell lysate (30 µg).
Lane 2: NEK6 knockout 293T whole cell lysate (30 µg).
ET7110-15 was shown to specifically react with NEK6 in wild-type 293T cells. No band was observed when NEK6 knockout sample was tested. Wild-type and NEK6 knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ET7110-15, 1/1,000) was used in 5% BSA at room temperature for 2 hours.
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