ATP2A1 / SERCA1 Recombinant Rabbit Monoclonal Antibody [JE45-75]
Catalog# ET7109-43
ATP2A1 / SERCA1 Recombinant Rabbit Monoclonal Antibody [JE45-75]
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WB
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IP
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IHC-P
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Human
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unconjugated
概述
产品名称
ATP2A1 / SERCA1 Recombinant Rabbit Monoclonal Antibody [JE45-75]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Synthetic peptide within Human SERCA1 ATPase aa 1-50 / 1,001.
种属反应性
Human
验证应用
WB, IP, IHC-P
分子量
Predicted band size: 110 kDa
阳性对照
Human fetal skeletal muscle tissue lysate, human fetal skeletal muscle tissue, human striated muscle tissue.
偶联
unconjugated
克隆号
JE45-75
RRID
产品特性
形态
Liquid
浓度
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
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WB
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1:500-1:1,000
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IP
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1:10-1:50
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IHC-P
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1:50-1:400
靶点
功能
This gene encodes one of the SERCA Ca(2+)-ATPases, which are intracellular pumps located in the sarcoplasmic or endoplasmic reticula of muscle cells. This enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen, and is involved in muscular excitation and contraction. Mutations in this gene cause some autosomal recessive forms of Brody disease, characterized by increasing impairment of muscular relaxation during exercise. Alternative splicing results in three transcript variants encoding different isoforms. There are 3 major domains on the cytoplasmic face of SERCA: the phosphorylation and nucleotide-binding domains, which form the catalytic site, and the actuator domain, which is involved in the transmission of major conformational changes. It seems that, in addition to the calcium-transporting properties, SERCA1 generates heat in some adipocytes and can improve cold tolerance in some wood frogs.
背景文献
1. Zhang Y et al. Characterization of cDNA and genomic DNA encoding SERCA1, the Ca(2+)-ATPase of human fast-twitch skeletal muscle sarcoplasmic reticulum, and its elimination as a candidate gene for Brody disease. Genomics 30:415-424 (1995).
2. Odermatt A et al. The mutation of Pro(789) to Leu reduces the activity of the fast-twitch skeletal muscle sarco(endo)plasmic reticulum Ca(2+) ATPase (SERCA1) and is associated with Brody disease. Hum Genet 106:482-491 (2000).
序列相似性
Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIA subfamily.
组织特异性
Skeletal muscle, fast twitch muscle (type II) fibers.
亚细胞定位
Endoplasmic reticulum membrane, Sarcoplasmic reticulum membrane.
UNIPROT
别名
fast twitch skeletal muscle isoform antibody
AT2A1_HUMAN antibody
ATP2A antibody
ATP2A1 antibody
ATPase Ca++ transporting cardiac muscle fast twitch 1 antibody
ATPase Ca++ transporting fast twitch 1 antibody
ATPase, Ca(2+)-transporting fast twitch 1 antibody
Calcium pump 1 antibody
Calcium transporting ATPase sarcoplasmic reticulum type fast twitch skeletal muscle isoform antibody
Calcium-transporting ATPase sarcoplasmic reticulum type antibody
展开图片
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Western blot analysis of SERCA1 ATPase on human fetal skeletal muscle tissue lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:500 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
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Immunohistochemical analysis of paraffin-embedded human fetal skeletal muscle tissue using anti-SERCA1 ATPase antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (ET7109-43) at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.
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Immunohistochemical analysis of paraffin-embedded human striated muscle tissue with Rabbit anti-ATP2A1 / SERCA1 antibody (ET7109-43) at 1/400 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-43) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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