Involucrin Recombinant Rabbit Monoclonal Antibody [JU30-27]
Catalog# ET1706-32
Involucrin Recombinant Rabbit Monoclonal Antibody [JU30-27]
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WB
-
IHC-P
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FC
-
Human
-
Rat
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unconjugated
概述
产品名称
Involucrin Recombinant Rabbit Monoclonal Antibody [JU30-27]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Synthetic peptide within Human Involucrin aa 131-180 / 585.
种属反应性
Human, Rat
验证应用
WB, IHC-P, FC
分子量
120 kDa
阳性对照
Human skin tissue lysates, human lung carcinoma tissue, A431.
偶联
unconjugated
克隆号
JU30-27
RRID
产品特性
形态
Liquid
浓度
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
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WB
-
1:500-1:2,000
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IHC-P
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1:50-1:200
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FC
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1:50-1:200
靶点
功能
Involucrin is a precursor protein of the keratinocyte cornified envelope, which is formed beneath the inner surface of the cell membrane during terminal differentiation. During keratinocyte terminal differentiation, glutamine residues of involucrin become covalently cross-linked to other envelope precursors via covalent epsilon-(gamma-glutamyl) lysine bonds. Moreover, its large size allows involucrin to cross-link molecules that are separated by substantial distances in the cornified envelope. These properties allow a single involucrin molecule to form multiple cross-links, in multiple spatial planes, with other envelope precursors.
背景文献
1. Dziunycz PJ et al. CYFIP1 is directly controlled by NOTCH1 and down-regulated in cutaneous squamous cell carcinoma. PLoS One 12:e0173000 (2017).
2. Kim BR et al. SOX2 and PI3K Cooperate to Induce and Stabilize a Squamous-Committed Stem Cell Injury State during Lung Squamous Cell Carcinoma Pathogenesis. PLoS Biol 14:e1002581 (2016).
序列相似性
Belongs to the involucrin family.
组织特异性
Keratinocytes of epidermis and other stratified squamous epithelia.
翻译后修饰
Substrate of transglutaminase. Some glutamines and lysines are cross-linked to other involucrin molecules, to other proteins such as keratin, desmoplakin, periplakin and envoplakin, and to lipids like omega-hydroxyceramide.
亚细胞定位
Cytoplasm.
别名
INVO_HUMAN antibody
Involucrin antibody
IVL antibody
图片
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Western blot analysis of Involucrin on human skin tissue lysates with Rabbit anti-Involucrin antibody (ET1706-32) at 1/500 dilution.
Lysates/proteins at 20 µg/Lane.
Predicted band size: 120 kDa
Observed band size: 120 kDa
Exposure time: 2 minutes;
8% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1706-32) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue with Rabbit anti-Involucrin antibody (ET1706-32) at 1/50 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1706-32) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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