Apg3 Recombinant Rabbit Monoclonal Antibody [JU00-35]
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Catalog# ET1706-29
Apg3 Recombinant Rabbit Monoclonal Antibody [JU00-35]
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WB
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IHC-P
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Human
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Mouse
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Rat
概述
产品名称
Apg3 Recombinant Rabbit Monoclonal Antibody [JU00-35]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Synthetic peptide within Human Apg3 1-50 / 314.
种属反应性
Human, Mouse, Rat
验证应用
WB, IHC-P
分子量
40 kDa
阳性对照
K562 cell lysate, HL-60 cell lysate, Hela cell lysate, Jurkat cell lysate, human tonsil tissue, human thyroid gland tissue, human colon carcinoma tissue, mouse small intestine tissue.
偶联
unconjugated
克隆号
JU00-35
RRID
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
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WB
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1:500-1:2,000
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IHC-P
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1:50-1:200
靶点
功能
Atg3 (autophagy-related protein 3), also known as APG3-like, hAPG3 or PC3-96, is an E2-like enzyme that localizes to the cytoplasm and is expressed in a variety of tissues with predominant levels found in kidney, placenta, liver, heart and skeletal muscle. Atg3 catalyzes the formation of the Atg8-phosphatidylethanolamine (Atg8-PE) conjugate, a reaction that is essential for autophagy (a cellular process that allows for the degradation of organelles and bulk cellular proteins). The process of forming the Atg8-PE conjugate begins with the removal of the C-terminal arginine residue of Atg8 by Atg4, a cysteine protease. The, now exposed, glycine residue is then activated by Atg7 and is then transferred to Atg3 for the final conjugation to PE. This last step can be accelerated by the presence of the Atg12-Atg5 conjugate which functions similarly to an E3 enzyme.
背景文献
1. Russ DW et al. Muscle-specificity of age-related changes in markers of autophagy and sphingolipid metabolism. Biogerontology 16:747-59 (2015).
2. Haldar AK et al. The E2-like conjugation enzyme Atg3 promotes binding of IRG and Gbp proteins to Chlamydia- and Toxoplasma-containing vacuoles and host resistance. PLoS One 9:e86684 (2014).
序列相似性
Belongs to the ATG3 family.
组织特异性
Widely expressed, with a highest expression in heart, skeletal muscle, kidney, liver and placenta.
翻译后修饰
Conjugated to ATG12 at Lys-243. ATG12-conjugation plays a role in regulation of mitochondrial homeostasis and cell death, while it is not involved in PE-conjugation to ATG8-like proteins and autophagy.; Cleaved by CASP8 upon death ligand binding such as tumor necrosis factor-alpha. CASP8 cleavage blocks survival-related autophagy and favors apoptosis.
亚细胞定位
Cytoplasm.
别名
2610016C12Rik antibody
Apg 3 antibody
APG3 antibody
APG3 autophagy 3 like antibody
APG3 like antibody
APG3, S. cerevisiae, homolog of antibody
APG3-like antibody
APG3L antibody
Apg3p antibody
ATG 3 antibody
展开图片
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Western blot analysis of Apg3 on different lysates with Rabbit anti-Apg3 antibody (ET1706-29) at 1/500 dilution.
Lane 1: Mouse testis tissue (20 µg/Lane)
Lane 2: K562 cell lysate (10 µg/Lane)
Lane 3: HL-60 cell lysate (10 µg/Lane)
Lane 4: Hela cell lysate (10 µg/Lane)
Lane 5: Jurkat cell lysate (10 µg/Lane)
Predicted band size: 40 kDa
Observed band size: 40 kDa
Exposure time: 2 minutes;
10% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1706-29) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/5,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-Apg3 antibody (ET1706-29) at 1/100 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1706-29) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human thyroid gland tissue with Rabbit anti-Apg3 antibody (ET1706-29) at 1/100 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1706-29) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-Apg3 antibody (ET1706-29) at 1/100 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1706-29) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue with Rabbit anti-Apg3 antibody (ET1706-29) at 1/100 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1706-29) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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