DDAH1 Recombinant Rabbit Monoclonal Antibody [JM53-18]
Catalog# ET1705-71
DDAH1 Recombinant Rabbit Monoclonal Antibody [JM53-18]
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WB
-
IP
-
Human
-
unconjugated
概述
产品名称
DDAH1 Recombinant Rabbit Monoclonal Antibody [JM53-18]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein within Human DDAH1 aa 100-285 / 285.
种属反应性
Human
验证应用
WB, IP
分子量
Predicted band size: 31 kDa
阳性对照
SH-SY5Y cell lysate, HepG2 cell lysate, HUVEC cell lysate, human prostate tissue, human kidney tissue.
偶联
unconjugated
克隆号
JM53-18
RRID
产品特性
形态
Liquid
浓度
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
-
1:5,000
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IP
-
Use at an assay dependent concentration.
靶点
功能
DDAH, a dimethylarginine dimethylaminohydrolase, hydrolyzes dimethyl arginine (ADMA) and monomethyl arginine (MMA), both inhibitors of nitric oxide synthases, and may be involved in in-vivo modulation of nitric oxide production. Impairment of DDAH causes ADMA accumulation and a reduction in cGMP generation. DDAH II, the predominant DDAH isoform in endothelial cells, facilitates the induction of nitric oxide synthesis by all-trans-Retinoic acid (atRA). DDAH proteins are highly expressed in colon, kidney, stomach and liver tissues.
背景文献
1. Wang H et al. DDAH1 plays dual roles in PM2.5 induced cell death in A549 cells. Biochim Biophys Acta 1860:2793-801 (2016).
2. Jung CS et al. Endogenous nitric-oxide synthase inhibitor ADMA after acute brain injury. Int J Mol Sci 15:4088-103 (2014).
序列相似性
Belongs to the DDAH family.
组织特异性
Detected in brain, liver, kidney and pancreas, and at low levels in skeletal muscle.
亚细胞定位
Cytoplasm.
UNIPROT
别名
DDAH antibody
DDAH I antibody
DDAH-1 antibody
DDAH1 antibody
DDAH1_HUMAN antibody
DDAHI antibody
Dimethylargininase 1 antibody
Dimethylargininase-1 antibody
Dimethylarginine dimethylaminohydrolase 1 antibody
N(G) antibody
展开图片
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☑ Knockdown (KD)
Western blot analysis of DDAH1 on different lysates with Rabbit anti-DDAH1 antibody (ET1705-71) at 1/5,000 dilution.
Lane 1: HAP1-parental cell lysate
Lane 2: HAP1-DDAH1 KD cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 31 kDa
Observed band size: 37 kDa
Exposure time: 20 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1705-71) at 1/5,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of DDAH1 on different lysates with Rabbit anti-DDAH1 antibody (ET1705-71) at 1/5,000 dilution.
Lane 1: SH-SY5Y cell lysate
Lane 2: HepG2 cell lysate
Lane 3: HUVEC cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 31 kDa
Observed band size: 37 kDa
Exposure time: 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1705-71) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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