GABARAP Recombinant Rabbit Monoclonal Antibody [JM30-30]
-
-
-
-
-
1+
Catalog# ET1705-53
GABARAP Recombinant Rabbit Monoclonal Antibody [JM30-30]
-
WB
-
IHC-P
-
Human
-
Mouse
-
Rat
概述
产品名称
GABARAP Recombinant Rabbit Monoclonal Antibody [JM30-30]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Synthetic peptide within Human GABARAP aa 10-59 / 117.
种属反应性
Human, Mouse, Rat
验证应用
WB, IHC-P
分子量
Predicted band size: 14 kDa
阳性对照
HeLa cell lysate, SH-SY5Y cell lysate, mouse kidney tissue lysate, rat liver tissue lysate, human placenta tissue, human kidney tissue, human pancreas tissue.
偶联
unconjugated
克隆号
JM30-30
RRID
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
-
1:2,000
-
IHC-P
-
1:50-1:200
靶点
功能
Gamma-aminobutyric acid receptor-associated protein is a protein that in humans is encoded by the GABARAP gene. Gamma-aminobutyric acid A receptors [GABA(A) receptors] are ligand-gated chloride channels that mediate inhibitory neurotransmission. This gene encodes GABA(A) receptor-associated protein, which is highly positively charged in its N-terminus and shares sequence similarity with light chain-3 of microtubule-associated proteins 1A and 1B. This protein clusters neurotransmitter receptors by mediating interaction with the cytoskeleton. Moreover, GABARAP has an important function in autophagosome mediated autophagy, since it is crucial for autophagosome formation and sequestration of cytosolic cargo into double-membrane vesicles, leading to subsequent degradation after fusion with lysosomes. In addition, GABARAP can mediate selective autophagy because it binds to so-called autophagic receptors (e.g. p62, NBr1), which bind and recruit specific cargo.
背景文献
1. Sanwald JL. et. al. The GABARAP Co-Secretome Identified by APEX2-GABARAP Proximity Labelling of Extracellular Vesicles. Cells. 2020 Jun
2. Agrotis A. et. al. Redundancy of human ATG4 protease isoforms in autophagy and LC3/GABARAP processing revealed in cells. Autophagy. 2019 Jun
序列相似性
Belongs to the ATG8 family.
组织特异性
Heart, brain, placenta, liver, skeletal muscle, kidney and pancreas.
翻译后修饰
The precursor molecule is cleaved by ATG4B to form the cytosolic form, GABARAP-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, GABARAP-II.
亚细胞定位
Endomembrane system. Cytoplasm, cytoskeleton.
别名
ATG8A antibody
FLC 3B antibody
FLC3B antibody
FLJ25768 antibody
GABA type A receptor associated protein antibody
GABA(A) receptor associated protein antibody
GABA(A) receptor-associated protein antibody
GABARAP a antibody
GABARAP antibody
Gamma aminobutyric acid receptor associated protein antibody
展开图片
-
Western blot analysis of GABARAP on different lysates with Rabbit anti-GABARAP antibody (ET1705-53) at 1/2,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: SH-SY5Y cell lysate
Lysates/proteins at 30 µg/Lane.
Predicted band size: 14 kDa
Observed band size: 16 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1705-53) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of GABARAP on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1705-53, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Mouse kidney tissue lysate
Lane 2: Rat liver tissue lysate -
Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-GABARAP antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-53, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
-
Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-GABARAP antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-53, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
-
Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-GABARAP antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-53, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"