Axin2 Recombinant Rabbit Monoclonal Antibody [JM11-30]
Catalog# ET1703-96
Axin2 Recombinant Rabbit Monoclonal Antibody [JM11-30]
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WB
-
IHC-P
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Human
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Mouse
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Rat
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unconjugated
概述
产品名称
Axin2 Recombinant Rabbit Monoclonal Antibody [JM11-30]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Synthetic peptide within Human Axin2 aa 55-93 / 843.
种属反应性
Human, Mouse, Rat
验证应用
WB, IHC-P
分子量
Predicted band size: 94 kDa
阳性对照
HepG2 cell lysate, HeLa cell lysate, SW480 cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, human colon cancer tissue, mouse colon tissue.
偶联
unconjugated
克隆号
JM11-30
RRID
产品特性
形态
Liquid
浓度
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
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WB
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1:500-1:2,000
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IHC-P
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1:50
靶点
功能
b-catenin is a component of both the cadherin cell adhesion system and the Wnt signaling pathway. Wnt signaling increases the amount of b-catenin, by preventing its ubiquitination and degradation, allowing its direct interaction with transcription factors of the lymphoid enhancer factor-T cell factor family and modulation of gene expression. Axin is involved in the degradation of b-catenin by acting as a scaffold to form a complex between b-catenin, adenomatous polyposis coli (APC) and GSK-3b. APC, which is phosphorylated by GSK-3b, induces degradation of b-catenin, thus inhibiting Wnt signal transduction. Conductin is 45% identical to axin and appears to play a similar role to axin in the Wnt signaling pathway.
背景文献
1. Ng OH et al. Deregulated WNT signaling in childhood T-cell acute lymphoblastic leukemia. Blood Cancer J 4:e192 (2014).
2. Winkler T et al. Wnt signaling activates Shh signaling in early postnatal intervertebral discs, and re-activates Shh signaling in old discs in the mouse. PLoS One 9:e98444 (2014).
组织特异性
Expressed in brain and lymphoblast.
翻译后修饰
Probably phosphorylated by GSK3B and dephosphorylated by PP2A.; ADP-ribosylated by tankyrase TNKS and TNKS2. Poly-ADP-ribosylated protein is recognized by RNF146, followed by ubiquitination and subsequent activation of the Wnt signaling pathway.; Ubiquitinated by RNF146 when poly-ADP-ribosylated, leading to its degradation and subsequent activation of the Wnt signaling pathway. Deubiquitinated by USP34, deubiquitinated downstream of beta-catenin stabilization step: deubiquitination is important Wnt signaling to positively regulate beta-catenin (CTNBB1)-mediated transcription.
亚细胞定位
Cytoplasm.
别名
Axil antibody
Axin like protein antibody
Axin-2 antibody
Axin-like protein antibody
Axin2 antibody
AXIN2_HUMAN antibody
Axis inhibition protein 2 antibody
Conductin antibody
DKFZp781B0869 antibody
MGC10366 antibody
展开图片
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Western blot analysis of Axin2 on different lysates with Rabbit anti-Axin2 antibody (ET1703-96) at 1/500 dilution.
Lane 1: HepG2 cell lysate (30 µg/Lane)
Lane 2: HeLa cell lysate (30 µg/Lane)
Lane 3: SW480 cell lysate (30 µg/Lane)
Lane 4: NIH/3T3 cell lysate (30 µg/Lane)
Lane 5: PC-12 cell lysate (30 µg/Lane)
Predicted band size: 94 kDa
Observed band size: 94 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1703-96) at 1/500 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-Axin2 antibody (ET1703-96) at 1/50 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-96) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse colon tissue with Rabbit anti-Axin2 antibody (ET1703-96) at 1/50 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-96) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
引文
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Fragment growth-based discovery of novel TNIK inhibitors for the treatment of colorectal cancer
期刊: European Journal Of Medicinal Chemistry
DOI: 10.1016/j.ejmech.2024.116240
IF: 6
应用: WB
反应种属: Human
发表时间: 2024 Feb
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The mechanism of exosomes of BMSCs modified with Bu Shen Yi Sui capsule in promoting remyelination via regulating miR-15b/Wnt signaling pathway-mediated differentiation of oligodendrocytes
期刊: Journal Of Ethnopharmacology
DOI:
IF: 4.8
应用: WB
反应种属: Mouse
发表时间: 2024 Dec
-
TROAP regulates cell cycle and promotes tumor progression through Wnt/β-Catenin signaling pathway in glioma cells. CNS neuroscience & therapeutics, 10.1111/cns.13688. Advance online publication.
期刊: CNS Neuroscience & Therapeutics
DOI:
IF: 4.019
应用: IF,IF-cell
反应种属: Human
发表时间: 2021 Jun
-
Long noncoding RNA expression profiles in intermittent parathyroid hormone induced cementogenesis. Genomics, 113(1 Pt 1), 217–228.
期刊: Genomics
DOI:
IF: 6.205
应用:
反应种属:
发表时间: 2021 Jan
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CUX1 Facilitates the Development of Oncogenic Properties Via Activating Wnt/β-Catenin Signaling Pathway in Glioma
期刊: Frontiers In Molecular Biosciences
DOI:
IF: 5
应用: WB,IF
反应种属: Human
发表时间: 2021 Aug
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