IgA Recombinant Rabbit Monoclonal Antibody [JM10-42]
Catalog# ET1703-10
IgA Recombinant Rabbit Monoclonal Antibody [JM10-42]
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WB
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IF-Cell
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IF-Tissue
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IHC-P
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IP
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Human
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unconjugated
概述
产品名称
IgA Recombinant Rabbit Monoclonal Antibody [JM10-42]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant full length protein of Human IgA aa 1-353 / 353.
种属反应性
Human
验证应用
WB, IF-Cell, IF-Tissue, IHC-P, IP
分子量
Predicted band size: 38 kDa
阳性对照
Human plasma lysates, HepG2, human tonsil tissue, human spleen tissue.
偶联
unconjugated
克隆号
JM10-42
RRID
产品特性
形态
Liquid
浓度
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
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WB
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1:1,000
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IF-Cell
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1:50-1:200
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IF-Tissue
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1:50-1:200
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IHC-P
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1:50-1:200
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IP
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Use at an assay dependent concentration.
靶点
功能
Immunoglobulin A (IgA, also referred to as sIgA in its secretory form) is an antibody that plays a role in the immune function of mucous membranes. The amount of IgA produced in association with mucosal membranes is greater than all other types of antibody combined. In absolute terms, between three and five grams are secreted into the intestinal lumen each day. This represents up to 15% of total immunoglobulins produced throughout the body. IgA has two subclasses (IgA1 and IgA2) and can be produced as a monomeric as well as a dimeric form. The IgA dimeric form is the most prevalent and is also called secretory IgA (sIgA). sIgA is the main immunoglobulin found in mucous secretions, including tears, saliva, sweat, colostrum and secretions from the genitourinary tract, gastrointestinal tract, prostate and respiratory epithelium. It is also found in small amounts in blood. The secretory component of sIgA protects the immunoglobulin from being degraded by proteolytic enzymes; thus, sIgA can survive in the harsh gastrointestinal tract environment and provide protection against microbes that multiply in body secretions. sIgA can also inhibit inflammatory effects of other immunoglobulins. IgA is a poor activator of the complement system, and opsonizes only weakly.
背景文献
1. Fahrbach KM et al. Differential binding of IgG and IgA to mucus of the female reproductive tract. PLoS One 8:e76176 (2013).
2. Kang S., et al. In situ identification and localization of IGHA2 in the breast tumor microenvironment by mass spectrometry. J. Proteome Res. 11:4567-4574(2012).
翻译后修饰
3-Hydroxykynurenine, an oxidized tryptophan metabolite that is common in biological fluids, reacts with alpha-1-microglobulin to form heterogeneous polycyclic chromophores including hydroxanthommatin. The chromophore reacts with accessible cysteines forming non-reducible thioether cross-links with Ig alpha-1 chain C region Cys-352.; N- and O-glycosylated. N-glycan at Asn-144: Hex5HexNAc4.
亚细胞定位
Cell membrane, Secreted.
别名
Hepatocellular carcinoma-associated protein TB6 antibody
Ig alpha 1 chain C region antibody
Ig alpha 2 chain C region antibody
IGHA antibody
IGHA1 antibody
IGHA2 antibody
Immunoglobulin heavy constant alpha 1 antibody
Immunoglobulin heavy constant alpha 2 A2m marker antibody
Immunoglobulin heavy constant alpha 2 antibody
PIgR antibody
展开图片
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Western blot analysis of IgA on human plasma lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1703-10, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Predicted band size: 38 kDa
Observed band size: 60 kDa -
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-IgA antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-10, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-IgA antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-10, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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