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Thymidine Kinase 1 / TK1 Recombinant Rabbit Monoclonal Antibody [JF0970]

RMB: 900 特惠 1500

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Catalog# ET1702-31

Thymidine Kinase 1 / TK1 Recombinant Rabbit Monoclonal Antibody [JF0970]

Application

  • WB

  • IF-Tissue

  • IHC-P

Reactivity

  • Human

This product has been cited in peer reviewed publications, see list HERE

概述

产品名称

Thymidine Kinase 1 / TK1 Recombinant Rabbit Monoclonal Antibody [JF0970]

抗体类型

Recombinant Rabbit monoclonal Antibody

免疫原

Synthetic peptide within Human Thymidine Kinase 1 aa 2-49 / 234.

种属反应性

Human

验证应用

WB, IF-Tissue, IHC-P

分子量

Predicted band size: 25 kDa

阳性对照

HeLa cell lysate, A549 cell lysate, human tonsil tissue.

偶联

unconjugated

克隆号

JF0970

RRID

AB_3070293

产品特性

形态

Liquid

存放说明

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

存储缓冲液

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

亚型

IgG

纯化方式

Protein A affinity purified.

应用稀释度

  • WB

  • 1:1,000

  • IF-Tissue

  • 1:50-1:200

  • IHC-P

  • 1:1,000

发表文章中的应用

WB 查看 1 篇文献如下

发表文章中的种属

Human 查看 1 篇文献如下

靶点

功能

Thymidine Kinase (TK1) is a highly conserved phosphotransferase that is present in most living cells. Thymidine Kinase catalyzes the phosphorylation reaction: deoxythymidine + ATP = deoxythymidine 5'-phosphate + ADP; it is thus involved in the reaction chain to introduce deoxythymidine into the DNA. Thymidine kinase is required for the action of many antiviral drugs, such as azidothymidine (AZT), and is is also used to select hybridoma cell lines in the production of monoclonal antibodies. Thymidine Kinase has many clinical applications as it is only present in anticipation of cell division. Because of this, Thymidine Kinase can be used as a proliferation marker in the diagnosis, treatment, and follow-up of malignant diseases, especially hematological malignancies. Thymidine Kinase may be observed as a monomer, dimer, trimer or tetramer.

背景文献

1. Breitbach CJ et al. Oncolytic vaccinia virus disrupts tumor-associated vasculature in humans. Cancer Res 73:1265-75 (2013).

2. Viel T et al. Early assessment of the efficacy of temozolomide chemotherapy in experimental glioblastoma using [18F]FLT-PET imaging. PLoS One 8:e67911 (2013).

序列相似性

Belongs to the thymidine kinase family.

翻译后修饰

Phosphorylated on Ser-13 in mitosis.

亚细胞定位

Cytoplasm.

UNIPROT

SwissProt: P04183 Human

别名

cytosolic antibody

KITH_HUMAN antibody

Thymidine kinase 1 antibody

Thymidine kinase 1 soluble antibody

Thymidine kinase 1 soluble isoform antibody

Thymidine kinase antibody

Thymidine kinase cytosolic antibody

TK 1 antibody

TK1 antibody

图片

  • <span style="font-weight: bold;">☑ Knockdown (KD)</span><br /><br />Western blot analysis of Thymidine Kinase 1 / TK1 on different lysates with Rabbit anti-Thymidine Kinase 1 / TK1 antibody (<a href="/products/ET1702-31" style="font-weight: bold;text-decoration: underline;">ET1702-31</a>) at 1/1,000 dilution.<br /><br />Lane 1: A549-si NT cell lysate<br />Lane 2: A549-si Thymidine Kinase 1 / TK1 cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 25 kDa<br />Observed band size: 25 kDa<br /><br />Exposure time: 45 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1702-31" style="font-weight: bold;text-decoration: underline;">ET1702-31</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Knockdown (KD)

    Western blot analysis of Thymidine Kinase 1 / TK1 on different lysates with Rabbit anti-Thymidine Kinase 1 / TK1 antibody (ET1702-31) at 1/1,000 dilution.

    Lane 1: A549-si NT cell lysate
    Lane 2: A549-si Thymidine Kinase 1 / TK1 cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 25 kDa
    Observed band size: 25 kDa

    Exposure time: 45 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1702-31) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of Thymidine Kinase 1 / TK1 on different lysates with Rabbit anti-Thymidine Kinase 1 / TK1 antibody (<a href="/products/ET1702-31" style="font-weight: bold;text-decoration: underline;">ET1702-31</a>) at 1/1,000 dilution.<br /><br />Lane 1: HeLa cell lysate<br />Lane 2: A549 cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 25 kDa<br />Observed band size: 25 kDa<br /><br />Exposure time: 1 minute; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1702-31" style="font-weight: bold;text-decoration: underline;">ET1702-31</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Thymidine Kinase 1 / TK1 on different lysates with Rabbit anti-Thymidine Kinase 1 / TK1 antibody (ET1702-31) at 1/1,000 dilution.

    Lane 1: HeLa cell lysate
    Lane 2: A549 cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 25 kDa
    Observed band size: 25 kDa

    Exposure time: 1 minute; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1702-31) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-Thymidine Kinase 1 / TK1 antibody (<a href="/products/ET1702-31" style="font-weight: bold;text-decoration: underline;">ET1702-31</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1702-31" style="font-weight: bold;text-decoration: underline;">ET1702-31</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-Thymidine Kinase 1 / TK1 antibody (ET1702-31) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-31) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

引文

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