14-3-3 gamma Recombinant Rabbit Monoclonal Antibody [SD20-65]
Catalog# ET1612-9
14-3-3 gamma Recombinant Rabbit Monoclonal Antibody [SD20-65]
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WB
-
IHC-P
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FC
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Human
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Mouse
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Rat
预测的种属支持售后
-
unconjugated
概述
产品名称
14-3-3 gamma Recombinant Rabbit Monoclonal Antibody [SD20-65]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Synthetic peptide within Human 14-3-3 gamma aa 131-180 / 247.
种属反应性
Human (Predicted: Mouse, Rat)
预测的种属支持售后
验证应用
WB, IHC-P, FC
分子量
Predicted band size: 28 kDa
阳性对照
293T cell lysate, K562 cell lysate, A431 cell lysate, Hela cell lysate, K562, human breast cancer tissue, human lung cancer tissue, human ovary cancer tissue.
偶联
unconjugated
克隆号
SD20-65
RRID
产品特性
形态
Liquid
浓度
存放说明
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
存储缓冲液
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
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WB
-
1:1,000-1:2,000
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IHC-P
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1:200-1:1,000
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FC
-
1:10-1:50
靶点
功能
14-3-3 proteins regulate many cellular processes relevant to cancer biology, notably apoptosis, mitogenic signaling and cell-cycle checkpoints. Seven isoforms comprise this family of signaling intermediates, denoted 14-3-3 b, g, e, z, h, q and s. 14-3-3 proteins form dimers that present two binding sites for ligand proteins, thereby bringing together two proteins that may not otherwise associate. These ligands largely share a 14-3-3 consensus binding motif and exhibit serine/threonine phosphorylation. 14-3-3 proteins function in broad regulation of these ligand proteins; by cytoplasmic sequestration, occupation of interaction domains and import/export sequences, prevention of degradation, activation/repression of enzymatic activity, and facilitation of protein modification. Loss of expression contributes to a vast array of pathogenic cellular activities.
背景文献
1. Scheibner, KA. et al. 2012. MiR-27a functions as a tumor suppressor in acute leukemia by regulating 14-3-3θ. PLoS ONE. 7: e50895.
2. Song, Y. et al. 2012. Expression of 14-3-3γ in patients with breast cancer: correlation with clinicopathological features and prognosis. Cancer Epidemiol. 36: 533-536.
序列相似性
Belongs to the 14-3-3 family.
组织特异性
Highly expressed in brain, skeletal muscle, and heart.
翻译后修饰
Phosphorylated by various PKC isozymes.
亚细胞定位
Cytoplasm.
别名
14 3 3 gamma antibody
14 3 3 protein gamma antibody
14 3 3 protein gamma subtype antibody
14 3 3gamma antibody
14-3-3 protein gamma antibody
1433G_HUMAN antibody
3 monooxygenase/tryptophan 5 monooxgenase activation protein gamma polypeptide antibody
KCIP 1 antibody
KCIP-1 antibody
KCIP1 antibody
展开图片
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☑ Knockdown (KD)
Western blot analysis of 14-3-3 gamma on different lysates with Rabbit anti-14-3-3 gamma antibody (ET1612-9) at 1/2,000 dilution.
Lane 1: HAP1-parental cell lysate
Lane 2: HAP1-14-3-3 gamma KD cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 28 kDa
Observed band size: 28 kDa
Exposure time: 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1612-9) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of 14-3-3 gamma on different lysates with Rabbit anti-14-3-3 gamma antibody (ET1612-9) at 1/1,000 dilution.
Lane 1: 293T cell lysate
Lane 2: K562 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 28 kDa
Observed band size: 28 kDa
Exposure time: 2 minutes;
12% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1612-9) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of 14-3-3 gamma on different lysates with Rabbit anti-14-3-3 gamma antibody (ET1612-9) at 1/500 dilution.
Lane 1: A431 cell lysate
Lane 2: Hela cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 28 kDa
Observed band size: 28 kDa
Exposure time: 2 minutes;
12% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1612-9) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-14-3-3 gamma antibody (ET1612-9) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1612-9) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue with Rabbit anti-14-3-3 gamma antibody (ET1612-9) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1612-9) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue with Rabbit anti-14-3-3 gamma antibody (ET1612-9) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1612-9) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Flow cytometric analysis of 14-3-3 gamma was done on K562 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1612-9, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
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