HDAC10 Recombinant Rabbit Monoclonal Antibody [SD08-11]
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Catalog# ET1612-69
HDAC10 Recombinant Rabbit Monoclonal Antibody [SD08-11]
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WB
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IF-Cell
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IP
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Human
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Rat
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Mouse
预测的种属支持售后
概述
产品名称
HDAC10 Recombinant Rabbit Monoclonal Antibody [SD08-11]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Synthetic peptide within Human HDAC10 aa 339-388 / 669.
种属反应性
Human, Rat (Predicted: Mouse)
预测的种属支持售后
验证应用
WB, IF-Cell, IP
分子量
Predicted band size: 71 kDa
阳性对照
Rat brain tissue lysate, rat cerebellum tissue lysate, rat pancreas tissue lysate, Hela, A431, A549, human placenta tissue.
偶联
unconjugated
克隆号
SD08-11
RRID
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
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WB
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1:1,000
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IF-Cell
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1:50-1:200
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IHC-P
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1:50-1:200
靶点
功能
Histone deacetylases (HDACs) play an important role in the modification of chromatin structure and thus in the suppression and activation of transcription and cellular differentiation. There are 11 members in the HDAC family that are divided into four classes. Class I HDACs represent homologs of the yeast histone deacetylase Rpd3, class II HDACs share strong homology with the yeast histone deacetylase Hda1, class III HDACs are closely related to the yeast Sir2 protein and class IV HDACs comprise histone deacetylase 11 (HDAC11)-related enzymes. HDAC10, also known as HD10, is a member of the class II HDACs. It contains an N-terminal Hda1p-related catalytic domain and a unique C-terminal leucine-rich domain. HDAC10 is ubiquitously expressed and can shuttle between the cytoplasm and nucleus in response to celllular signals. It is able to repress transcription and, like other class II HDAC members, its enzymatic activity is inhibited by Trichostatin A (TSA).
背景文献
1. Liu J et al. Both HDAC5 and HDAC6 are required for the proliferation and metastasis of melanoma cells. J Transl Med 14:7 (2016).
2. Takase K et al. Monoaminergic and neuropeptidergic neurons have distinct expression profiles of histone deacetylases. PLoS One 8:e58473 (2013).
序列相似性
Belongs to the histone deacetylase family. HD type 2 subfamily.
组织特异性
Widely expressed with high levels in liver and kidney.
亚细胞定位
Cytoplasm, Nucleus.
别名
DKFZP761B039 antibody
HD 10 antibody
HD10 antibody
HDA10_HUMAN antibody
HDAC 10 antibody
Hdac10 antibody
Histone deacetylase 10 antibody
MGC149722 antibody
OTTHUMP00000028555 antibody
图片
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Western blot analysis of HDAC10 on different lysates with Rabbit anti-HDAC10 antibody (ET1612-69) at 1/500 dilution.
Lane 1: Rat brain tissue lysate
Lane 2: Rat cerebellum tissue lysate
Lane 3: Rat pancreas tissue lysate
Lysates/proteins at 40 µg/Lane.
Predicted band size: 71 kDa
Observed band size: 75 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1612-69) at 1/500 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
ICC staining of HDAC10 in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1612-69, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
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ICC staining of HDAC10 in A431 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1612-69, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
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ICC staining of HDAC10 in A549 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1612-69, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
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Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-HDAC10 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1612-69, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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